Fourier ptychographic microscopy (FPM) is a computational microscopy platform capable of large‐area, high‐resolution imaging of thin specimens. FPM enhances the spatial resolution beyond the diffraction limit of the objective by synthesizing frequency information from multiple images captured at various illumination angles. However, this enhancement comes at the cost of temporal resolution, making conventional FPMs unsuitable for imaging dynamic samples. The low temporal resolution of FPM is mainly attributed to two factors: the large number of measurements required for resolution improvement and the long exposure time, particularly in capturing dark‐field (DF) images. Here, a novel high‐throughput FPM strategy, termed PEH‐FPM, enabled by the combination of photon‐efficient, lenslet‐array‐integrated LED illuminator, and a hybrid multiplexed‐illumination scheme is introduced. The illuminator provides vignette‐free, high‐intensity illumination at the object plane, thereby dramatically reducing the DF acquisition time to a few milliseconds and eliminating vignetting‐induced artifacts present in conventional FPM approaches. The illumination‐multiplexing scheme significantly reduces the number of measurements, achieving a 0.51‐NA resolution across a field of view of 4.1 mm2 with a total acquisition time of 21–40 msec, using 4 × 0.13NA objective. The imaging platform is expected to find numerous applications that call for dynamic, high‐resolution imaging over a large spatial scale.

中文翻译：

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使用小透镜集成 LED 照明器进行高通量傅里叶叠层相位显微镜的光子高效混合照明


傅里叶叠层成像显微镜 （FPM） 是一种计算显微镜平台，能够对薄标本进行大面积、高分辨率成像。FPM 通过合成以各种照明角度捕获的多个图像的频率信息，将空间分辨率提高到物镜衍射极限之外。然而，这种增强是以牺牲时间分辨率为代价的，这使得传统 FPM 不适合对动态样品进行成像。FPM 的低时间分辨率主要归因于两个因素：提高分辨率所需的大量测量和较长的曝光时间，尤其是在捕获暗场 （DF） 图像时。在这里，介绍了一种新颖的高通量 FPM 策略，称为 PEH-FPM，通过结合光子高效、小透镜阵列集成 LED 照明器和混合多路复用照明方案来实现。该照明器在物体平面上提供无晕影的高强度照明，从而将 DF 采集时间大幅缩短至几毫秒，并消除了传统 FPM 方法中存在的暗角引起的伪影。照明多路复用方案显著减少了测量次数，使用 4 × 0.13NA 物镜，在 4.1 mm2 的视场内实现了 0.51-NA 的分辨率，总采集时间为 21-40 毫秒。预计该成像平台将发现许多需要在大空间尺度上进行动态、高分辨率成像的应用。