The efficient removal of Naphthalene (Nap), a persistent organic pollutant known for its severe endocrine-disrupting and carcinogenic properties, is vital for environmental protection. This study introduces a straightforward approach for addressing persistent organic pollutants in water through the strategic construction of a photo-enzyme coupled catalytic system (PECS). In this study, we fabricated a photocatalyst/enzyme heterojunction by immobilizing lipase (LPZYM) on a composite material of graphitic carbon nitride and zinc oxide (g-C₃N₄/ZnO, ZCN), which served as the photocatalytic substrate for the degradation of Nap. The resulting ZCN/LPZYM heterojunction demonstrated outstanding degradation efficacy and cyclic stability in the aqueous removal of Nap, achieving a degradation rate of 97.36 % under visible light irradiation within 150 min. This performance significantly surpasses that of g-C₃N₄ (64.16 %), ZnO (79.31 %), and ZCN alone (87.22 %). The superior degradation capabilities of ZCN/LPZYM can be primarily attributed to the synergistic catalytic effects of the enzyme, coupled with enhanced visible light absorption and carrier separation efficiency. Mechanistic investigations revealed that superoxide anion radicals (O₂^–), hydroxyl radicals (–OH), and singlet oxygen (¹O₂) play critical roles in the Nap degradation process, with the order of effectiveness being O₂^–> –OH > ¹O₂.

中文翻译：

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g-C3N4/ZnO 异质连接固定化脂肪酶高效协同催化降解水中萘


萘 （Nap） 是一种持久性有机污染物，以其严重的内分泌干扰和致癌特性而闻名，有效去除萘 （Nap） 对于环境保护至关重要。本研究介绍了一种通过战略性构建光酶偶联催化系统 （PECS） 来解决水中持久性有机污染物的简单方法。在这项研究中，我们通过在石墨氮化碳和氧化锌 （g-C ₃ N ₄ /ZnO， ZCN） 的复合材料上固定脂肪酶 （LPZYM） 制备了光催化剂/酶异质结，该复合材料作为光催化降解 Nap 的底物。所得的 ZCN/LPZYM 异质结在水去除 Nap 方面表现出优异的降解效能和循环稳定性，在可见光照射下 150 分钟内降解率达到 97.36 %。这一性能明显超过了 g-C ₃ N ₄ （64.16 %）、ZnO （79.31 %） 和单独的 ZCN （87.22 %）。ZCN/LPZYM 卓越的降解能力主要归因于酶的协同催化作用，以及增强的可见光吸收和载体分离效率。机理调查显示，超氧阴离子自由基 （O ₂ ^– ）、羟基自由基 （–OH） 和单线态氧 （ ¹ O ₂ ） 在 Nap 降解过程中起关键作用，有效性顺序为 O ₂ ^– > –OH > ¹ O ₂ 。