Covalent modification is an effective strategy for reducing the allergenicity of single allergens. However, due to the complexity of the food matrix, its application in hypoallergenic food production requires further exploration. The study showed that covalent modification of fucoidan decreased the specific antibody levels, inhibited Th2 cell differentiation, and reduced mast cell degranulation, suggesting that it significantly reduced the allergenicity of Penaeus vannamei. Further analysis showed that covalent modification not only up-regulated the intestinal tight junction proteins expression and improved intestinal mucus secretion but also restored intestinal microbial homeostasis by immunological and 16S rRNA gene sequencing. Additionally, covalent modification inhibited dendritic cell maturation and CD8⁺ T cell differentiation, thereby reducing antigen recognition and presentation, as determined by transcriptome and flow cytometry. Therefore, the covalent modification of fucoidan reduced shrimp allergenicity by enhancing intestinal barrier function and inhibiting antigen presentation. In conclusion, it is a potential strategy for processing hypoallergenic foods.

中文翻译：

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岩藻多糖介导的共价修饰通过增强肠道屏障功能和抗原呈递抑制来减轻虾 （Penaeus vannamei） 对小鼠的过敏性


共价修饰是降低单一过敏原致敏性的有效策略。然而，由于食品基质的复杂性，其在低过敏性食品生产中的应用需要进一步探索。研究表明，岩藻多糖的共价修饰降低了特异性抗体水平，抑制了 Th2 细胞分化，减少了肥大细胞脱颗粒，表明它显着降低了凡纳滨对虾的致敏性。进一步分析表明，共价修饰不仅上调了肠道紧密连接蛋白的表达，改善了肠道粘液分泌，而且通过免疫学和 16S rRNA 基因测序恢复了肠道微生物稳态。此外，共价修饰抑制树突状细胞成熟和 CD8 ⁺ T 细胞分化，从而减少抗原识别和呈递，如转录组和流式细胞术所确定的那样。因此，岩藻多糖的共价修饰通过增强肠道屏障功能和抑制抗原呈递来降低虾的致敏性。总之，它是加工低过敏性食品的潜在策略。