Manganese is essential for various biological functions; however, excessive exposure can lead to significant health risks, particularly brain neurotoxicity. Understanding manganese-induced alterations in brain serum protein levels and brain function is crucial for elucidating the mechanisms underlying manganese neurotoxicity. To address this, we developed a novel NIR-II fluorescent probe, RSM, characterized by robust binding to serum albumin and high sensitivity. Using RSM, we observed that heightened BSA uptake in cells exposed to elevated manganese concentrations relative to those exposed to lower levels. Furthermore, we successfully detected changes in serum albumin levels induced by manganese neurotoxicity in brain tissue through in situ NIR-II fluorescence imaging. Our findings establish an association between augmented manganese-induced neurotoxicity and elevated serum albumin content in the brain. This work provides a valuable tool for further investigating the mechanisms of toxic molecules.

中文翻译：

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开发新型 NIR-II 荧光探针，用于监测锰暴露诱导的血清白蛋白神经毒性波动


锰对各种生物功能至关重要;然而，过度接触会导致重大的健康风险，尤其是脑神经毒性。了解锰诱导的脑血清蛋白水平和脑功能改变对于阐明锰神经毒性的潜在机制至关重要。为了解决这个问题，我们开发了一种新型 NIR-II 荧光探针 RSM，其特点是与血清白蛋白的结合稳健且灵敏度高。使用 RSM，我们观察到相对于暴露于较低浓度锰的细胞，暴露于较高锰浓度的细胞中 BSA 摄取增加。此外，我们通过原位 NIR-II 荧光成像成功检测了锰神经毒性在脑组织中诱导的血清白蛋白水平的变化。我们的研究结果确定了锰诱导的神经毒性增加与大脑中血清白蛋白含量升高之间的关联。这项工作为进一步研究毒性分子的机制提供了有价值的工具。