Using quantitative proteomics, the study investigated the effects of low-frequency alternating magnetic field-assisted freezing (LF-MFF) on the oxidative status and structural integrity of porcine myofibrillar proteins (MPs). LF-MFF, especially at 3 mT (LF-MFF-3) and 4 mT (LF-MFF-4), significantly reduced MPs' oxidation compared to refrigerator freezing (RF) (P < 0.05). The spectroscopic analysis confirmed better structural preservation with LF-MFF-4. We identified 126 differentially abundant proteins (DAPs) associated with key metabolic pathways, including amino acid biosynthesis and oxidative phosphorylation, potentially affecting Adenosine Triphosphate (ATP) metabolism and contributing to freeze-induced protein damage and oxidative denaturation of MPs. Through correlation analysis, among the 52 DAPs in the LF-MFF-4 vs RF comparison, eight proteins with variable importance in projection (VIP) > 1.1 were identified as potential biomarkers for porcine MPs. These findings enhance our understanding of the oxidative and structural changes in MPs following LF-MFF, suggesting its potential for improving pork quality and meat preservation.

中文翻译：

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基于蛋白质组学变化的低频交变磁场辅助冷冻对猪肉肌原纤维蛋白氧化和结构特性的影响机制分析


该研究使用定量蛋白质组学研究了低频交变磁场辅助冷冻 （LF-MFF） 对猪肌原纤维蛋白 （MP） 氧化状态和结构完整性的影响。LF-MFF，尤其是 3 mT （LF-MFF-3） 和 4 mT （LF-MFF-4） 与冰箱冷冻 （RF） 相比，显著降低了 MPs 的氧化 （P < 0.05）。光谱分析证实 LF-MFF-4 的结构保留效果更好。我们鉴定了 126 种与关键代谢途径相关的差异丰度蛋白 （DAP），包括氨基酸生物合成和氧化磷酸化，可能影响三磷酸腺苷 （ATP） 代谢并导致冷冻诱导的蛋白质损伤和 MPs 的氧化变性。通过相关性分析，在 LF-MFF-4 与 RF 比较的 52 个 DAPs 中，8 个具有可变投影重要性 （VIP） > 1.1 的蛋白被鉴定为猪 MP 的潜在生物标志物。这些发现增强了我们对 LF-MFF 后 MPs 氧化和结构变化的理解，表明其在改善猪肉质量和肉类保存方面的潜力。