Despite having several advantages, bicontinuously structured polymeric nanoparticles (BSPNPs) are far less explored in the field of controlled drug delivery owing to the requirement of complex precursor copolymers and the associated multistep synthetic procedures. In this work, we report the synthesis of a redox-sensitive diblock copolymer (P1), which was subsequently utilized to prepare doxorubicin (DOX) containing a pH-labile prodrug (P2). P1 and P2 spontaneously self-assembled in aqueous media above their critical aggregation concentration, forming micellar nanoparticles with rare bicontinuous morphology that promotes loading of both hydrophobic and hydrophilic cargoes in different compartments. To the best of our knowledge, the formation of BSPNPs through direct self-assembly in aqueous media has not yet been reported. In vitro cellular studies asserted the higher safety profile of the nanoparticles against noncancerous cells (HEK293T) than free DOX, whereas they displayed higher drug-induced cytotoxicity against cancer cells (MCF-7) in comparison to free DOX, establishing them as promising cancer drug delivery systems.

中文翻译：

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来自嵌段共聚物前药在水性介质中的自组装的双连续纳米颗粒，用于潜在的癌症治疗


尽管具有多项优势，但由于需要复杂的前体共聚物和相关的多步合成程序，双连续结构聚合物纳米颗粒 （BSPNP） 在受控药物递送领域的探索要少得多。在这项工作中，我们报道了氧化还原敏感二嵌段共聚物 （P1） 的合成，随后用于制备含有 pH 不稳定前药 （P2） 的阿霉素 （DOX）。P1 和 P2 在高于其临界聚集浓度的水介质中自组装，形成具有罕见双连续形态的胶束纳米颗粒，可促进疏水和亲水货物在不同隔室中的负载。据我们所知，通过在水性介质中直接自组装形成 BSPNP 的报道尚未报道。体外细胞研究断言，纳米颗粒对非癌细胞 （HEK293T） 的安全性高于游离 DOX，而与游离 DOX 相比，它们对癌细胞 （MCF-7） 表现出更高的药物诱导细胞毒性，使其成为有前途的癌症药物递送系统。