Lactobacillus helveticus LZ-R-5 (R-5), a strain with high epithelial adhesion and bioactive exopolysaccharide production, was isolated from Tibetan kefir grains. This study investigated its potential to alleviate intestinal inflammation using a DSS-induced colitis model in BALB/c mice. We integrated microbial diversity and serological analyses to assess changes in gut flora and cytokines following the R-5 treatment. Pathological assessments showed that R-5 reduced crypt distortion in the proximal colon and mitigated hepatic immune challenges by enhancing gut barrier function. The increased relative expression of TGF-β1 and the downregulation of NLRP3-related inflammatory factors were conducive to preventing organ damage in the thymus and spleen of mice with colitis. Additionally, R-5 stimulated GPR43 expression and improved epithelial nutrition, promoting mucin production to prevent enterotoxin leakage. It also modulated the gut microbiota by suppressing Bacteroides and Erysipelatoclostridium, leading to a microbiota composition more akin to that of normal flora.

中文翻译：

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瑞士乳杆菌 LZ-R-5 通过调节肠道菌群和增强肠道屏障功能改善 DSS 诱导的小鼠结肠炎


瑞士乳杆菌LZ-R-5 （R-5） 是一种具有高上皮粘附性和生物活性胞外多糖产生的菌株，是从藏族开菲尔粒中分离得到的。本研究使用 DSS 诱导的 BALB/c 小鼠结肠炎模型调查了其缓解肠道炎症的潜力。我们整合了微生物多样性和血清学分析，以评估 R-5 处理后肠道菌群和细胞因子的变化。病理评估显示，R-5 通过增强肠道屏障功能减少了近端结肠的隐窝变形并减轻了肝脏免疫挑战。TGF-β1 相对表达增加和 NLRP3 相关炎症因子下调有利于防止结肠炎小鼠胸腺和脾脏器官损伤。此外，R-5 刺激 GPR43 表达并改善上皮营养，促进粘蛋白产生以防止肠毒素泄漏。它还通过抑制拟杆菌属和丹毒梭菌来调节肠道微生物群，导致微生物群组成更类似于正常菌群的组成。