Dietary fibers (DF) are important components of human and animal diets. However, they can decrease protein digestibility and absorption and thus the nutritional value of a food. The aim of this study was to investigate how the form of delivery of pea DF impacted the integrity of the intestinal barrier and, thereby, the potential absorption of molecules. To this end, two pea flours, with either intact or ruptured cell walls, and two controls, pea fibers and pea protein, were digested in vitro and the digesta obtained applied onto a jejunum porcine cell line (IPEC-J2 cells). Cell viability and integrity were evaluated by transepithelial electrical resistance measurement, colorimetric assay (MTS), and immunohistochemistry for tight junction proteins. Additionally, the diffusion of FITC-dextran (FD4) and lucifer yellow (LY) through the epithelial cell monolayers was monitored. The digested pea samples did not alter the IPEC-J2 viability and permeability. For instance, no difference in the diffusion of molecules either FD4 or LY across the monolayers was observed between the different digesta and the control. Similarly, no effect was observed in ZO-1 labeling intensity compared to the control. This study demonstrated that intestinal integrity was maintained whether pea cell walls were intact or ruptured.

中文翻译：

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暴露于消化豌豆成分后肠道屏障功能的体外评估 - 食物基质效应


膳食纤维 （DF） 是人类和动物饮食的重要组成部分。然而，它们会降低蛋白质的消化率和吸收率，从而降低食物的营养价值。本研究的目的是调查豌豆 DF 的递送形式如何影响肠道屏障的完整性，从而影响分子的潜在吸收。为此，在体外消化两种细胞壁完整或破裂的豌豆粉和两种对照，豌豆纤维和豌豆蛋白，并将获得的消化物应用于空肠猪细胞系（IPEC-J2 细胞）。通过跨上皮电阻测量、比色测定 （MTS） 和免疫组化评估紧密连接蛋白的细胞活力和完整性。此外，还监测了 FITC-葡聚糖 （FD4） 和荧光黄 （LY） 通过上皮细胞单层的扩散。消化的豌豆样品不改变 IPEC-J2 的活力和通透性。例如，在不同的消化膜和对照之间没有观察到 FD4 或 LY 分子在单层中的扩散差异。同样，与对照相比，未观察到 ZO-1 标记强度的影响。这项研究表明，无论豌豆细胞壁完整还是破裂，肠道完整性都能保持。