This work aimed to investigate the extraction of compounds from the byproduct of oil extraction (BOE) of forage radish seeds, using hydroethanolic solvent under pressurized liquid conditions. For this purpose, the influence of the variables temperature (100, 125 and 150 °C), pressure (50, 75 and 100 bar) and time (30 and 60 min) on the extraction process was evaluated. The chemical characterization, antibacterial activity, cytotoxicity and genotoxicity of the extracts was determined, as well as its purification. Temperature was the variable that most influenced the responses, resulting in greater overall yield (in terms of mass and active compounds) as it increased. A diverse phenolic profile was found in the extracts, encompassing different classes such as phenolic acids, flavonoids and aldehydes. The predominant compounds were sinapic, malic, nicotinic acids and rutin. The extract showed better antimicrobial activity against Streptococcus pyogenes with MIC value of 11.71 mg mL⁻¹. The concentrations of extracts evaluated were not cytotoxic or genotoxic to tissue with high cell proliferation. After purification with XAD 7HP resin, the content of phenolic compounds and the antioxidant capacity had a pronounced increase in the obtained product.

中文翻译：

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从饲料萝卜种子的油提取副产品中绿色提取有价值的化合物


本研究旨在研究在加压液体条件下使用水乙醇溶剂从饲料萝卜种子的油萃取副产品 （BOE） 中提取化合物。为此，评估了变量温度（100、125 和 150 °C）、压力（50、75 和 100 bar）和时间（30 和 60 min）对萃取过程的影响。测定提取物的化学特性、抗菌活性、细胞毒性和遗传毒性，并对其进行纯化。温度是影响响应最大的变量，随着温度的增加，总产量（就质量和活性化合物而言）更高。在提取物中发现了多种酚类物质，包括酚酸、类黄酮和醛类等不同类别。主要化合物是 sinapic、malic、烟酸和芦丁。该提取物对化脓性链球菌显示出更好的抗菌活性，MIC 值为 11.71 mg ^mL-1。评估的提取物浓度对高细胞增殖的组织没有细胞毒性或遗传毒性。用 XAD 7HP 树脂纯化后，所得产物中酚类化合物的含量和抗氧化能力显著增加。