Alcoholic beverages, such as wine, beer, and distilled spirits, are widely produced and consumed in different nations. Different types of alcoholic beverages contain different combinations of flavor compounds. However, rapid and simultaneous analysis of a large variety of compounds in alcoholic beverages by a miniatured and portable device remains a challenge. In this paper, a Mycobacterium smegmatis porin A (MspA) nanopore modified with a phenylboronic acid (PBA) adapter is applied for rapid analysis of a variety of alcoholic beverages. By utilizing custom machine learning algorithms, various cis-diols are identified simultaneously in both distilled and fermented alcoholic beverages, generating unique barcodes for each sample type. Nanopore analysis of alcoholic beverages has also never been carried out previously. Rapid grading of wine sweetness and detection of additives, including sucrose and D-tartaric acid, are also demonstrated, showcasing the significance of this technique in the administration of wine production.

中文翻译：

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主要酒精饮料的纳米孔特征


葡萄酒、啤酒和蒸馏酒等酒精饮料在不同国家广泛生产和消费。不同类型的酒精饮料包含不同的风味化合物组合。然而，使用小型便携式设备快速、同时分析酒精饮料中的多种化合物仍然是一项挑战。在本文中，用苯硼酸 （PBA） 接头修饰的耻垢分枝杆菌孔蛋白 A （MspA） 纳米孔用于各种酒精饮料的快速分析。通过利用自定义机器学习算法，可以同时识别蒸馏和发酵酒精饮料中的各种顺式二醇，从而为每种样品类型生成唯一的条形码。酒精饮料的纳米孔分析以前也从未进行过。还展示了葡萄酒甜度的快速分级和添加剂（包括蔗糖和 D-酒石酸）的检测，展示了该技术在葡萄酒生产管理中的重要性。