BACKGROUNDNeoseiulus californicus is a predatory mite that can control various spider mites and other small arthropods. Despite its acknowledged effectiveness in the natural enemy market, a crucial knowledge gap exists in understanding the genomic features related to its predatory traits and adaptation. With the increasing emphasis on modern pest management strategies and dynamic environmental changes in plant production trends, constructing a reliable genomic resource for N. californicus becomes imperative.RESULTSIn this study, we provided a high‐quality genome assembly and annotation of N. californicus, with a size of 188.43 Mb and 12 946 predicted genes. We identified genomic features and traits related to its detoxification, stress response, sensory system, mobility, secretory toxins, digestive enzymes and horizontally transferred genes (HGTs) by comparative genomics. We also predicted the potential off‐target effects of double‐stranded RNA (dsRNA) that could be used to control spider mites, based on public data and the N. californicus genome.CONCLUSIONOur study provided a valuable genomic resource for a commercialized predatory mite, offering useful insights for the design of integrated pest management (IPM) strategies in the new era. Further studies are needed to explore the functional roles of key gene families in predatory mites, as well as their interactions with their prey and IPM strategies. © 2024 Society of Chemical Industry.

中文翻译：

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捕食性螨类 Neoseiulus californicusMcGregor （Acari： Phytoseiidae） 的高质量参考基因组提供了对其生物学特性和潜在 RNAi 脱靶效应的见解


背景加利福尼亚螨虫是一种捕食性螨虫，可以控制各种蜘蛛螨和其他小型节肢动物。尽管它在天敌市场中的有效性得到公认，但在理解与其捕食性状和适应相关的基因组特征方面存在关键的知识差距。随着植物生产趋势中对现代害虫管理策略的日益重视和动态环境变化，构建可靠的加利福尼亚猪笼草基因组资源变得势在必行。结果在本研究中，我们提供了高质量的加利福尼亚猪笼草基因组组装和注释，大小为 188.43 Mb，预测基因为 12 946 个。我们通过比较基因组学鉴定了与其解毒、应激反应、感觉系统、活动性、分泌毒素、消化酶和水平转移基因 （HGT） 相关的基因组特征和性状。我们还根据公共数据和加利福尼亚猪笼草基因组预测了可用于控制蜘蛛螨的双链 RNA （dsRNA） 的潜在脱靶效应。结论本研究为商业化捕食性螨虫提供了宝贵的基因组资源，为新时代害虫综合治理 （IPM） 策略的设计提供了有用的见解。需要进一步的研究来探索关键基因家族在捕食性螨虫中的功能作用，以及它们与猎物和 IPM 策略的相互作用。© 2024 化工学会.