Shewanella oneidensis MR-1 is an electroactive bacterium commonly employed in the design of microbial fuel cells (MFCs) due to its ability to convert organic matter to electricity. Its applicability is limited by low adhesion to the surface of the electrode, which decreases the efficiency of charge transfer and reduces the available power outputs. In this study, we aimed to improve the adhesion, viability, and extracellular charge transfer ability of S. oneidensis on the surface of electrodes modified with poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS), which is a conducting polymer frequently used to enhance the performance of MFCs. PEDOT:PSS-coated glass surface was modified with organic moieties, namely, glucose, sucrose, maltose, cellulose, chitosan, poly(vinyl alcohol), poly-l-lysine, and laminin. The modified surfaces were then analyzed using Fourier-transform infrared spectroscopy, energy dispersive X-ray spectroscopy, electrochemical impedance spectroscopy, scanning electron microscopy, and fluorescence microscopy, and the results demonstrated an overall improvement in their chemical composition, substantial roughness, and moderate wettability. Biofilm formation was found to be significantly improved on PEDOT:PSS surface coated with glucose, resulting in 54.8 ± 1.2% increase in the amount of biomass. LIVE/DEAD analysis indicated a significantly higher percentage of live bacteria (97.5 ± 1.5%) on the PEDOT:PSS surface coated with glucose when compared to bare PEDOT:PSS (42.1 ± 2.1%). The improved adhesion of S. oneidensis on the glucose-functionalized PEDOT:PSS surface resulted in enhanced charge transfer characteristics, leading to significant decrease in charge transfer resistance at the electrode interface. Our approach shows promise in the further development of efficient renewable energy technology for bioelectricity generation.

中文翻译：

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表面功能化 PEDOT：PSS 界面用于改善 Shewanella oneidensis MR-1 的粘附、活力和细胞外电荷转移


鲷鲷MR-1 是一种电活性细菌，常用于微生物燃料电池 （MFC） 的设计，因为它能够将有机物转化为电能。其适用性受到对电极表面的低粘附力的限制，这会降低电荷转移的效率并降低可用的功率输出。在这项研究中，我们旨在提高 S. oneidensis 在用聚（3,4-乙烯二氧噻吩）:p oly（苯乙烯磺酸盐）（PEDOT：PSS） 改性的电极表面的粘附性、活力和细胞外电荷转移能力，这是一种经常用于增强 MFC 性能的导电聚合物。PEDOT：PSS 涂层玻璃表面用有机基团改性，即葡萄糖， 蔗糖、麦芽糖、纤维素、壳聚糖、聚（乙烯醇）、聚-L-赖氨酸和层粘连蛋白。然后使用傅里叶变换红外光谱、能量色散 X 射线光谱、电化学阻抗谱、扫描电子显微镜和荧光显微镜对改性表面进行分析，结果表明其化学成分、相当大的粗糙度和适度的润湿性都有所改善。发现在涂有葡萄糖的 PEDOT：PSS 表面生物膜形成显着改善，导致生物量增加 54.8 ± 1.2%。LIVE/DEAD 分析表明，与裸 PEDOT：PSS 相比，涂有葡萄糖的 PEDOT：PSS 表面上的活细菌百分比 （97.5 ± 1.5%） 显着更高 （42.1 ± 2.1%）。S 的粘附性得到改善。 葡萄糖功能化 PEDOT：PSS 表面的 oneidensis 导致电荷转移特性增强，导致电极界面处的电荷转移电阻显着降低。我们的方法在进一步开发用于生物发电的高效可再生能源技术方面显示出前景。