l-Homoserine is proven to be a promising precursor for the synthesis of l-phosphinothricin, which is a broad-spectrum herbicide with low toxicity. To enhance the sustainability of the process, the utilization of nonfood carbon sources for l-homoserine production is highly sought-after. Acetate, which can be captured from C1 gases, has the potential to become the most abundant feedstock for biomanufacturing. In this study, we systematically engineered the Escherichia coli strain to generate l-homoserine from acetate. The production of l-homoserine from acetate was initially achieved by deleting branch pathways, activating the glyoxylate shunt, and enhancing the synthetic pathway. The strain’s tolerance and acetate assimilation capabilities were strengthened through adaptive laboratory evolution. Reducing the flux toward gluconeogenesis further facilitated the production of l-homoserine. Finally, the engineered strain produced 15.96 g/L l-homoserine solely from CO₂-derived acetate and formate in a 5 L fermentor via a fed-batch process. This study provides insights into the development of a low-carbon bioeconomy for the herbicide industry.

中文翻译：

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通过工程大肠杆菌菌株仅从 CO2 衍生的乙酸盐和甲酸盐可持续生产 l-高丝氨酸


l-Homoserine 被证明是合成 l-phosphinothricin 的一种很有前途的前体，l-phosphinothricin 是一种毒性低的广谱除草剂。为了提高工艺的可持续性，利用非食品碳源生产 l-高丝氨酸备受追捧。可以从 C1 气体中捕获的乙酸盐有可能成为生物制造中最丰富的原料。在这项研究中，我们系统地设计了大肠杆菌菌株，以从乙酸盐中生成 l-高丝氨酸。从乙酸盐生产 l-高丝氨酸最初是通过删除分支途径、激活乙醛酸盐分流和增强合成途径来实现的。该菌株的耐受性和乙酸盐同化能力通过适应性实验室进化得到加强。减少糖异生的通量进一步促进了 l-高丝氨酸的产生。最后，工程菌株在 5 L 发酵罐中通过补料分批工艺仅从 CO₂ 衍生的乙酸盐和甲酸盐中生产了 15.96 g/L l-高丝氨酸。本研究为除草剂行业低碳生物经济的发展提供了见解。