Acute myocardial infarction (AMI) is one of the most common causes of sudden death in cardiovascular disease, and myoglobin (Myo) is the first protein to be released in the blood after the attack, which is an important biomarker for clinical detection of AMI. The “Golden Rescue Time” for acute myocardial infarction is to intervene within the first 30 min after the attack; therefore, a rapid and accurate Myo detection method is needed urgently. In this study, we designed a combined enzyme-linked immunosorbent assay (ELISA) technique with surface-enhanced Raman scattering (SERS) immunoassay (ELI-SERS), which integrates the small sample volume, ease of operation, and excellent linearity of ELISA while utilizing the SERS technique and selecting the molecule with the Raman signal (IR-808), which is in resonance with the excitation wavelength, for further signal enhancement. The sensitivity of the system was further improved by optimizing the key factors in the assay such as incubation time, particle concentration, and temperature. Compared with the sandwich-structured magnetic bead method, no collection and concentration steps are required, simplifying the operation and ultimately realizing a sensitivity of a 5.3 pg/mL antigen detectable in 6 min. In actual serum samples, we achieve 100% accuracy and sensitivity by adding blockers to exclude the effect of heterophilic antibodies in serum and to reduce false positives in blank samples. We also validated the hepatitis B surface antigen test, demonstrating the universality of our system. Overall, this study designed an ultrasensitive and convenient SERS sensor for the detection of Myo, which extends the practical application of SERS and also contributes methods for the detection of other biomarkers.

中文翻译：

---

基于 ELI-SERS 构建一种方便且高灵敏度的血清中 Myo 检测传感器


急性心肌梗死 （AMI） 是心血管疾病中最常见的猝死原因之一，肌红蛋白 （Myo） 是发作后血液中首先释放的蛋白质，是临床检测 AMI 的重要生物标志物。急性心肌梗死的“黄金抢救时间”是在发作后的前 30 分钟内进行干预;因此，迫切需要一种快速准确的 Myo 检测方法。在这项研究中，我们设计了一种酶联免疫吸附测定 （ELISA） 技术与表面增强拉曼散射 （SERS） 免疫测定 （ELI-SERS） 相结合，该技术结合了 ELISA 的小样本量、易操作性和出色的线性度，同时利用 SERS 技术并选择具有拉曼信号 （IR-808） 的分子，与激发波长共振，以进一步增强信号。通过优化分析中的关键因素（如孵育时间、颗粒浓度和温度），进一步提高了系统的灵敏度。与夹心结构磁珠法相比，无需收集和浓缩步骤，简化了操作，并最终实现了 6 分钟内可检测到 5.3 pg/mL 抗原的灵敏度。在实际血清样品中，我们通过添加阻断剂来排除血清中嗜异性抗体的影响并减少空白样品中的假阳性，从而实现 100% 的准确性和灵敏度。我们还验证了乙型肝炎表面抗原检测，证明了我们系统的普遍性。综上所述，本研究设计了一种超灵敏、便捷的 SERS 传感器用于检测 Myo，扩展了 SERS 的实际应用，也为其他生物标志物的检测提供了方法。