Development of a reliable tool to detect hydrogen peroxide (H₂O₂) and rutin in food-derived products and bioactive flavonoids is essential for food safety. Nevertheless, food/drug-based real samples are complex matrices that affect the sensor's specificity and sensitivity. For this purpose, we developed a simple electrochemical detection platform using covalent organic framework‑silver nanoparticles (COF-AgNPs). Based on spectral and electrochemical tests, COF-AgNPs displayed enhanced electroactive sites and facile electron transfer. For H₂O₂ and rutin, the designed sensor surface exhibited outstanding concentration linearity of 0.5 nM–1000 μM and 1 nM–900 μM, respectively, along with superior detection limits of 0.126 nM and 0.133 nM. Additionally, it demonstrated acceptable reproducibility and interference capability. In practical analysis, H₂O₂ and rutin were detected in milk, fruits, and drug samples with high recovery rates of 94.60–99.31 % (n = 3). Consequently, the designed sensor is ideal for screening targets for H₂O₂ and rutin in food sources and for food research.

中文翻译：

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制备杂化纳米复合材料，用于真实水果和药物样品中食品防腐剂和过氧化氢和芦丁的生物活性靶标的电化学评价


开发一种可靠的工具来检测食品衍生产品中的过氧化氢 （H ₂ O ₂ ） 和芦丁以及生物活性类黄酮对于食品安全至关重要。然而，基于食品/药物的真实样品是影响传感器特异性和灵敏度的复杂基质。为此，我们开发了一种使用共价有机框架-银纳米颗粒 （COF-AgNPs） 的简单电化学检测平台。基于光谱和电化学测试，COF-AgNPs 显示出增强的电活性位点和电子传输。对于 H ₂ O ₂ 和 rutin，设计的传感器表面分别表现出 0.5 nM–1000 μM 和 1 nM–900 μM 的出色浓度线性，以及 0.126 nM 和 0.133 nM 的出色 LOD。此外，它还展示了可接受的可重复性和干扰能力。在实际分析中，在牛奶、水果和药物中检测到 H ₂ O ₂ 和芦丁，回收率高达 94.60-99.31 % （n = 3）。因此，所设计的传感器非常适合筛选食品来源中的 H ₂ O ₂ 和芦丁靶标以及食品研究。