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A highly prevalent and specific cryptic plasmid pBI143 for human fecal pollution tracking in a subtropical urban river
Water Research ( IF 11.4 ) Pub Date : 2024-12-16 , DOI: 10.1016/j.watres.2024.122992
Yawen Liu, Wendy J.M. Smith, Metasebia Gebrewold, Rory Verhagen, Stephen Cook, Stuart L. Simpson, Bethany Oceguera Johnson, Kyle Bibby, Warish Ahmed

Microbial source tracking (MST) is a critical tool for identifying sources of human and animal fecal pollution in aquatic environments. To enhance human fecal pollution tracking, this study evaluated the performance characteristics of pBI143, a cryptic plasmid recently identified for potential MST applications. Nucleic acid samples from ten animal species were screened for pBI143, revealing its presence in a small number of pigs, cows, dogs, cats, and flying fox fecal samples. Despite minor cross-detection with non-human fecal samples, pBI143 exhibited a high specificity value (up to 0.93). In untreated urban wastewater, pBI143 was consistently detected in all samples, exhibiting higher concentrations than the well-established human Bacteroides HF183 marker gene. Following a wastewater discharge event, pBI143 concentrations were monitored in an urban river and correlated well with both HF183 and enterococci 23S rRNA marker genes. Using conditional probability analysis, the likelihood of human fecal pollution was estimated to be 89.3 % when pBI143 was detected in 50 % of the river water samples. This study demonstrates that pBI143 is a highly abundant and specific human fecal marker for tracking human fecal pollution in environmental waters. Monitoring of pBI143 could significantly improve the accuracy of human fecal source identification in environmental waters, offering valuable insights for public health risk management and pollution mitigation strategies.

中文翻译:


一种高度普遍且特异性的隐蔽质粒 pBI143,用于亚热带城市河流中人类粪便污染追踪



微生物源追踪 (MST) 是识别水生环境中人类和动物粪便污染源的关键工具。为了增强人类粪便污染追踪,本研究评估了 pBI143 的性能特征,pBI143 是一种最近确定用于潜在 MST 应用的隐蔽质粒。对 10 种动物的核酸样本进行了 pBI143 筛查,揭示了它存在于少量猪、牛、狗、猫和狐蝠粪便样本中。尽管与非人粪便样本进行了轻微的交叉检测,但 pBI143 表现出高特异性值 (高达 0.93)。在未经处理的城市污水中,在所有样品中一致检测到 pBI143,其浓度高于公认的人类拟杆菌 HF183 标记基因。废水排放事件后,监测城市河流中的 pBI143 浓度,并与 HF183 和肠球菌 23S rRNA 标记基因密切相关。使用条件概率分析,当在 50% 的河水样本中检测到 pBI143 时,人类粪便污染的可能性估计为 89.3%。本研究表明,pBI143 是一种高度丰富且特异的人类粪便标志物,用于追踪环境水域中的人类粪便污染。监测 pBI143 可以显著提高环境水域中人类粪便来源识别的准确性,为公共卫生风险管理和污染缓解策略提供有价值的见解。
更新日期:2024-12-16
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