The present study was conducted to evaluate the impact of α‐Cypermethrin (αCYP), the second most commonly used pesticide in India, on the ovine ovarian granulosa cells (GCs) viability, growth, apoptosis, and steroidogenesis. GCs collected from abattoir‐derived ovine ovaries were cultured for 3/6 days in the presence of various concentrations of αCYP (0, 1, 10, 25, 50, and 100 μM). The results revealed a binary effect on GCs, where metabolic activity and viability rates were significantly (p < 0.05) lower from 25 μM onwards. Estrogen concentration was significantly low from the 1 μM dose, whereas progesterone concentration showed a significant increase (10 μM) in the spent media of cultured GCs. The cytotoxicity in the GCs exposed to αCYP revealed significant changes in LDH, ROS, CUPRAC, and GST activity (all at 25 μM) and MDA (at 10 μM) compared to those observed in the control group. The gene expression profiles of cultured GCs showed a significant up‐regulation of CYP11A1, FSHR (all at 1 μM), StAR, BAX, and CASP3 (all at 10 μM), 3βHSD1 (at 25 μM), and significant down‐regulation of CYP17A1 and ERS2 (all at 25 μM), CYP19A1 and 17βHSD (all at 1 μM), ESR1 and BCL2 (all at 10 μM) in comparison to those observed in control groups. The results of the present experiment demonstrated that αCYP affected the growth and functional parameters of GCs, the expression of steroid hormone‐associated genes, and hormone secretion.

中文翻译：

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α氯氰菊酯诱导的绵羊卵巢颗粒细胞功能障碍的生化和分子级联反应


本研究旨在评估印度第二常用杀虫剂 α-氯氰菊酯 （αCYP） 对绵羊卵巢颗粒细胞 （GCs） 活力、生长、细胞凋亡和类固醇生成的影响。从屠宰场衍生的绵羊卵巢收集的 GC 在不同浓度的 αCYP（0、1、10、25、50 和 100 μM）存在下培养 3/6 天。结果揭示了对 GC 的二元效应，其中代谢活性和活力率从 25 μM 开始显着降低 （p < 0.05）。雌激素浓度从 1 μM 剂量开始显着降低，而黄体酮浓度显示培养 GC 的用过培养基显着增加 （10 μM）。与对照组相比，暴露于 αCYP 的 GC 中的细胞毒性显示 LDH、ROS、CUPRAC 和 GST 活性（均为 25 μM）和 MDA（10 μM）发生显著变化。培养的 GC 的基因表达谱显示 CYP11A1、FSHR（均为 1 μM）、StAR、BAX 和 CASP3（均为 10 μM）、3βHSD1（25 μM）显著上调，CYP17A1 和 ERS2（均为 25 μM）、CYP19A1 和 17βHSD（均为 1 μM）、ESR1 和 BCL2（均为 10 μM）与对照组相比显著下调。本实验结果表明，αCYP 影响 GCs 的生长和功能参数、类固醇激素相关基因的表达和激素分泌。