Peri-implantitis, caused by bacterial biofilm on dental implants, leads to bone loss and tissue inflammation, ultimately causing oral health decline. Traditional methods to remove biofilm are ineffective in promoting reosseointegration on implant surfaces. This phenomenon can be attributed to two factors: incomplete removal of biofilm from hard-to-reach areas and alterations in the physicochemical properties of implant surfaces caused by decontamination procedures. To address this problem, we developed D-arginine-loaded chitooligosaccharide-capped pH-responsive mesoporous silica nanoparticles (Dar@MSN-COS) for improving the efficacy of decontamination of Water Jet (WJ). Dar@MSN-COS particles exhibit a targeted approach towards the extracellular polymeric substance (EPS) in order to disrupt the biofilm, and possess the capability to infiltrate confined areas between implant screws. Following this, the WJ treatment effectively removed residual biofilm and demonstrated improved cleaning efficacy. Furthermore, the decontamination of the Dar@MSN-COS combination with WJ promotes effective cell cytocompatibility on the titanium surface. The results of mechanistic experiments indicate that Dar@MSN-COS may act on biofilms by releasing a significant quantity of reactive oxygen species (ROS), suggesting it as a key contributing factor. In summary, our novel therapeutic protocol shows promise as an alternative solution for addressing the clinically complex aspects of peri-implantitis.

中文翻译：

---

负载 D-精氨酸的 pH 响应介孔二氧化硅纳米颗粒增强了水射流疗法在净化生物膜涂层钛表面方面的功效。


种植体周围炎是由种植牙上的细菌生物膜引起的，导致骨质流失和组织炎症，最终导致口腔健康下降。去除生物膜的传统方法在促进植入物表面的骨整合方面无效。这种现象可归因于两个因素：难以触及区域的生物膜未完全去除以及去污程序引起的植入物表面物理化学性质的改变。为了解决这个问题，我们开发了载有 D-精氨酸的壳寡糖封端的 pH 响应介孔二氧化硅纳米颗粒 （Dar@MSN-COS），用于提高水射流 （WJ） 净化的效果。Dar@MSN-COS 颗粒表现出对细胞外聚合物物质 （EPS） 的靶向方法，以破坏生物膜，并具有渗透到植入螺钉之间的受限区域的能力。此后，WJ 处理有效去除残留的生物膜，并显示出更高的清洁效果。此外，Dar@MSN-COS 与 WJ 组合的去污促进了钛表面的有效细胞细胞相容性。机理实验结果表明，Dar@MSN-COS 可能通过释放大量活性氧 （ROS） 作用于生物膜，表明它是一个关键的促成因素。总之，我们的新型治疗方案有望成为解决种植体周围炎临床复杂方面的替代解决方案。