This study investigates the transcriptional profile of a novel oil-degrading microbial consortium (MC1) composed of four bacterial isolates from Brazilian oil reservoirs: Acinetobacter baumannii subsp. oleum ficedula, Bacillus velezensis, Enterobacter asburiae, and Klebsiella pneumoniae. Genomic analysis revealed an enrichment of genes associated with xenobiotic degradation, particularly for aminobenzoate, atrazine, and aromatic compounds, compared to reference genomes. The consortium demonstrated superior growth and complete oil degradation relative to individual strains. Transcriptional profiling during growth on oil indicated that key subsystems involved membrane transport, stress response, and dehydrogenase complexes, crucial for hydrocarbon uptake. Notably, genes for degrading aromatics, naphthalene, and chloroalkanes were significantly expressed during the initial oil growth phase. The dominant gene expressed was alkane 1-monooxygenase, particularly in the late growth phase. While A. baumannii exhibited the highest transcriptional activity, B. velezensis showed lower activity despite possessing numerous hydrocarbon degradation genes. The synergistic interactions among strains, confirmed by complementary gene expression patterns, position MC1 as a promising bioremediation agent for hydrocarbon-contaminated environments. However, more than collaboration, competition for nutrient uptake and resistance to stress drive gene expression and adaptation in the presence of oil as the carbon source.

中文翻译：

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解锁油性废物降解细菌联盟的转录谱


本研究调查了由来自巴西油藏的四种细菌分离物组成的新型石油降解微生物联盟 （MC1） 的转录谱：鲍曼不动杆菌亚种 oleum ficedula、芽孢杆菌 velezensis、Enterobacter asburiae 和肺炎克雷伯菌.基因组分析显示，与参考基因组相比，与外源性生物降解相关的基因富集，特别是氨基苯甲酸盐、阿特拉津和芳香族化合物。相对于单个菌株，该联盟表现出优异的生长和完全的油降解。在油上生长过程中的转录分析表明，关键子系统涉及膜转运、应激反应和脱氢酶复合物，这对碳氢化合物吸收至关重要。值得注意的是，降解芳烃、萘和氯烷烃的基因在油的初始生长期显著表达。表达的优势基因是烷烃 1-单加氧酶，尤其是在生长期晚期。虽然鲍曼不动杆菌表现出最高的转录活性，但 B. velezensis 尽管拥有许多碳氢化合物降解基因，但表现出较低的活性。菌株之间的协同相互作用，通过互补基因表达模式得到证实，使 MC1 成为一种有前途的碳氢化合物污染环境生物修复剂。然而，在以石油为碳源的情况下，对营养吸收和抗逆性的竞争不仅推动了基因表达和适应。