This study examined nine prominent commercially available single-cell RNA sequencing (scRNA-seq) kits across four technology groups. Each kit was characterized using peripheral blood mononuclear cells (PBMCs) from a single donor, which enabled consistent assessment of factors such as analytical performance, protocol duration and cost. The Chromium Fixed RNA Profiling kit from 10× Genomics, with its probe-based RNA detection method, demonstrated the best overall performance. The Rhapsody WTA kit from Becton Dickinson exhibited a balance between performance and cost. Importantly, we introduce the read utilization metric, which differentiates scRNA-seq kits based on the efficiency of converting sequencing reads into usable counts. Thus, read utilization is an important feature that substantially impacts sensitivity and cost. With data from 169, 262 cells, our work provides a comprehensive comparison of commercial scRNA-seq technologies to facilitate the effective implementation of single-cell studies.

中文翻译：

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用于评估商业单细胞 RNA 测序技术的综合分析框架


本研究检查了四个技术组中的 9 个著名的市售单细胞 RNA 测序 （scRNA-seq） 试剂盒。使用来自单个供体的外周血单核细胞 （PBMC） 对每个试剂盒进行表征，从而能够对分析性能、方案持续时间和成本等因素进行一致评估。10× Genomics 的 Chromium 固定 RNA 分析试剂盒采用基于探针的 RNA 检测方法，表现出最佳的整体性能。Becton Dickinson 的 Rhapsody WTA 套件在性能和成本之间取得了平衡。重要的是，我们引入了读取利用率指标，该指标根据将测序读数转换为可用计数的效率来区分 scRNA-seq 试剂盒。因此，读取利用率是一项重要功能，它对灵敏度和成本有重大影响。凭借来自 169、262 个细胞的数据，我们的工作提供了商业 scRNA-seq 技术的全面比较，以促进单细胞研究的有效实施。