Lactobacillus acidophilus L. acidophilus Lactobacillus, Bifidobacterium, and Akkermansia, This study aimed to explore the ameliorative effects and underlying mechanisms of oral administration Lactobacillus acidophilus 6074 fermented jujube juice (LAFJ) on dextran sulfate sodium (DSS)‐induced colitis in mice. In this study, jujube juice was used as a substrate and fermented by L. acidophilus 6074 to investigate its effects on gut microbiota, intestinal barrier function, oxidative stress, inflammatory factors, and short‐chain fatty acids (SCFAs) in mice with colitis and to reveal its potential mechanism for alleviating colitis. The results demonstrated that fermentation caused significant changes in the nutrients and nonnutrients of jujube juice, mainly in organic acids (malic acid, lactic acid, citric acid, and succinic acid) and free amino acids (Thr, Met, Ser, Ile, and Lys). High‐dose LAFJ (20 mL/kg/day) significantly reduced the disease activity index (DAI), improved histopathological morphology, and increased colon length in colitis mice. LAFJ alleviated colon damage and preserved the integrity of the colonic mucosal barrier by promoting the expression of colonic tight junction proteins occludin, claudin‐1, and zonula occluden‐1 (ZO‐1). Furthermore, LAFJ inhibited the production of proinflammatory factors and attenuated oxidative stress. Gut microbiota of mice revealed that LAFJ increased beneficial bacteria such as Lactobacillus, Bifidobacterium, and Akkermansia, promoted the production of SCFAs, and inhibited the growth of harmful microorganisms. Overall, LAFJ could reshape and restore gut microbiota imbalance caused by intestinal inflammation and alleviate the development of colitis, which may become a novel dietary intervention.

中文翻译：

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嗜酸乳杆菌 6074 发酵枣汁通过修复肠道屏障、调节炎症因子和肠道菌群改善 DSS 诱导的结肠炎


嗜酸乳杆菌 L.嗜酸杆菌乳酸菌、双歧杆菌和阿克曼氏菌，本研究旨在探讨口服嗜酸乳杆菌 6074 发酵枣汁 （LAFJ） 对葡聚糖硫酸钠 （DSS） 诱导的小鼠结肠炎的改善作用和潜在机制。本研究以红枣汁为底物，经嗜酸乳杆菌 6074 发酵，探讨其对结肠炎小鼠肠道菌群、肠道屏障功能、氧化应激、炎症因子和短链脂肪酸 （SCFAs） 的影响，揭示其缓解结肠炎的潜在机制。结果表明，发酵导致枣汁营养成分和非营养成分发生显著变化，主要为有机酸（苹果酸、乳酸、柠檬酸和琥珀酸）和游离氨基酸（Thr、Met、Ser、Ile 和 Lys）。高剂量 LAFJ （20 mL/kg/d） 显著降低结肠炎小鼠的疾病活动指数 （DAI），改善组织病理形态，并增加结肠长度。LAFJ 通过促进结肠紧密连接蛋白 occludin、claudin-1 和 zonula occluden-1 （ZO-1） 的表达来减轻结肠损伤并保持结肠粘膜屏障的完整性。此外，LAFJ 抑制促炎因子的产生并减轻氧化应激。小鼠肠道菌群显示，LAFJ 增加了乳酸菌、双歧杆菌和阿克曼西亚等有益菌，促进了 SCFA 的产生，并抑制了有害微生物的生长。总体而言，LAFJ 可以重塑和恢复肠道炎症引起的肠道菌群失衡，并缓解结肠炎的发展，这可能成为一种新颖的饮食干预措施。