Background

Benzodiazepine abuse remains a significant public health concern. Current sample preparation methods for benzodiazepine analysis from human serum often involve complex procedures that require large sample volumes and extensive organic solvent use. To address these limitations, this study presents a novel and efficient sample preparation method utilizing 3D-printed sorbent devices.

Results

The 3D-printed devices, fabricated from a thermoplastic composite incorporating C18-modified silica, demonstrated exceptional performance in extracting benzodiazepines from human serum. The method was optimized and validated according to ICH guidelines, ensuring its reliability for quantitative benzodiazepine analysis. Notably, the method required minimal sample and solvent volumes, eliminating the need for protein precipitation, evaporation, and reconstitution.

Significance

This novel sample preparation approach offers significant advantages over traditional methods, providing a more efficient and environmentally friendly solution for benzodiazepine analysis. The versatility of 3D printing allows for the customization of sorbent devices for various analytes and matrices, expanding the potential applications of this method. Coupled with a rapid and robust LC-MS method optimized with DryLab, this approach presents a valuable and sensitive tool for benzodiazepine monitoring in clinical and toxicological settings.

中文翻译：

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一种用于人血清中苯二氮卓类药物定量的新型 3D 打印样品制备方法

 背景

苯二氮卓类药物滥用仍然是一个重大的公共卫生问题。目前用于人血清苯二氮卓类药物分析的样品制备方法通常涉及复杂的程序，需要大量样品和大量使用有机溶剂。为了解决这些限制，本研究提出了一种利用 3D 打印吸附剂装置的新型高效样品制备方法。

 结果

这些 3D 打印设备由含有 C18 改性二氧化硅的热塑性复合材料制成，在从人血清中提取苯二氮卓类药物方面表现出卓越的性能。该方法根据 ICH 指南进行了优化和验证，确保了其定量苯二氮卓类药物分析的可靠性。值得注意的是，该方法需要极少的样品和溶剂体积，无需蛋白质沉淀、蒸发和重构。

 意义

与传统方法相比，这种新颖的样品制备方法具有显著优势，为苯二氮卓类药物分析提供了更高效、更环保的解决方案。3D 打印的多功能性允许为各种分析物和基质定制吸附剂装置，从而扩展了该方法的潜在应用。该方法与使用 DryLab 优化的快速、稳定的 LC-MS 方法相结合，为临床和毒理学环境中的苯二氮卓类药物监测提供了一种有价值且灵敏的工具。