In the post-harvest process of the chrysanthemums, the fading of the capitulum is severe because of the significant reduction of anthocyanin content at the later full-bloom stage, making the selection of appropriate post-harvest preservative essential for color retention and enhancement. Jasmonates (JAs) have positive significance in promoting the accumulation of plant pigments and the synthesis of secondary metabolites and delaying plant senescence, thus they are widely used as a new type of eco-friendly post-harvest preservative in many fruits. In the present study, the flower color of the chrysanthemum cultivar 'Riqie Taohong' deepened under the spraying treatment of methyl jasmonate (MeJA), accompanied by the increase of metabolite concentrations of anthocyanins and JAs as well as the expression of several genes involved in their biosynthetic and signaling pathway in the ray florets at the early capitulum developmental stage. The yeast two-hybrid and bimolecular fluorescence complementation assays showed that CmJAZ7 (jasmonate ZIM domain 7) strongly interacted with positive regulators CmMYB6/7 (v-myb avian myeloblastosis 6/7), which suppressed the activity of CmMYB6/7 and anthocyanin biosynthesis. Based on the above results, the molecular mechanism of exogenous MeJA application on the regulation of anthocyanin biosynthesis in chrysanthemum was supposed, which provided a reference for revealing the molecular mechanism of MeJA on the regulation of flower color in ornamental plants, as well as for enhancing the flower color of commercial flowers using harmless plant hormones at the post-harvest stage.

中文翻译：

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CmJAZ7 和 CmMYB6/7 之间的相互作用影响茉莉酸甲酯诱导的菊花萌小花中花青素积累


在菊花采后过程中，由于盛花后期花青素含量显著降低，花序褪色严重，选择合适的采后防腐剂对于保色和增强显色至关重要。茉莉酸酯 （JAs） 在促进植物色素积累和次生代谢产物合成、延缓植物衰老方面具有积极意义，因此在许多水果中被广泛用作一种新型的环保采后防腐剂。本研究以茉莉酸甲酯（methyl jasmonate，MeJA）喷施处理后，菊花品种'日奇桃红'的花色加深，伴随着花色苷和JAs代谢物浓度的增加，以及头状花序发育早期花序小花中参与其生物合成和信号通路的几个基因的表达。酵母双杂交和双分子荧光互补试验显示，CmJAZ7 （jasmonate ZIM 结构域 7） 与正调节因子 CmMYB6/7 （v-myb 禽成髓细胞增多症 6/7） 强烈相互作用，从而抑制 CmMYB6/7 和花青素生物合成的活性。基于以上结果，推测了外源 MeJA 应用对菊花中花青素生物合成调控的分子机制，为揭示 MeJA 对观赏植物花色调控的分子机制，以及在收获后利用无害植物激素增强商品花卉的花色提供参考。