SULTR1;1 and SULTR1;2 are high-affinity sulphate transporters that are crucial for sulphate uptake from the soil. Nevertheless, the impact of sulphate absorption mediated by SULTR1;1 and SULTR1;2 on the metabolism of sulfur-containing compounds as well as the physiological processes they are involved in is not yet fully understood. In this study, we cloned the BoSULTR1;1 and BoSULTR1;2 genes from broccoli (Brassica oleracea var. italica). Overexpression of BoSULTR1;1/BoSULTR1;2 in Arabidopsis enhanced sulphate uptake and assimilation, without accumulating the sulphate assimilation product cysteine. Overexpression of BoSULTR1;1/BoSULTR1;2 also triggered the upregulation of genes responsible for the biosynthesis of another two sulfur-containing compounds, methionine and glutathione, without leading to their accumulation, rather, it led to the accumulation of the downstream secondary metabolites, glucosinolates. Under normal growth conditions, BoSULTR1;1 and BoSULTR1;2 are specifically expressed in the roots. However, certain treatments such as wounding, PEG 6000-simulated drought stress, and methyl jasmonate can induce the expression of the two genes in the aerial parts, indicating that BoSULTR1;1/BoSULTR1;2 may not only regulate sulfur uptake but also potentially control the distribution of sulphate in the above-ground tissues and participate in defense against certain adverse environmental cffigonditions. Given that glucosinolates are crucial defense compounds against both biotic and abiotic stresses, we detected stress tolerance in plants overexpressing BoSULTR1;1/BoSULTR1;2. The results revealed that overexpression of BoSULTR1;1/BoSULTR1;2 in Arabidopsis significantly enhanced resistance to Helicoverpa armigera, bacteria pathogen Pseudomonas syringae pv. tomato DC3000 and drought stress. Similar results were observed when BoSULTR1;1/BoSULTR1;2 was overexpressed in broccoli, indicating a conservative role of BoSULTR1;1/BoSULTR1;2 in Brassicaceae plants. In conclusion, BoSULTR1;1 and BoSULTR1;2 are effective candidate genes for enhancing sulfur uptake and improving stress resistance in Brassicaceae plants such as broccoli. Their potential applications in agricultural production are highly promising.

中文翻译：

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BoSULTR1 的过表达;1/博舒尔特 1;2 促进西兰花的硫代葡萄糖苷积累并增强抗逆性


苏尔特1;1 和 SULTR1;2 是高亲和力硫酸盐转运蛋白，对于土壤中硫酸盐的吸收至关重要。然而，SULTR1 介导的硫酸盐吸收的影响;1 和 SULTR1;2 关于含硫化合物的代谢以及它们参与的生理过程尚不完全清楚。在这项研究中，我们克隆了 BoSULTR1;1 和 BoSULTR1;来自西兰花 （Brassica oleracea var. italica） 的 2 个基因。BoSULTR1 的过表达;1/博舒尔特 1;拟南芥中的 2 增强了硫酸盐的吸收和同化，而不会积累硫酸盐同化产物半胱氨酸。BoSULTR1 的过表达;1/博舒尔特 1;2 还触发了负责另外两种含硫化合物蛋氨酸和谷胱甘肽生物合成的基因的上调，但没有导致它们的积累，而是导致下游次生代谢物硫代葡萄糖苷的积累。在正常生长条件下，BoSULTR1;1 和 BoSULTR1;2 在根中特异性表达。然而，某些处理如伤伤、PEG 6000 模拟干旱胁迫和茉莉酸甲酯可以诱导这两个基因在地上部分的表达，表明 BoSULTR1;1/博舒尔特 1;2 不仅可以调节硫的吸收，还可以潜在地控制硫酸盐在地上组织中的分布，并参与防御某些不利的环境变化。鉴于硫代葡萄糖苷是对抗生物和非生物胁迫的重要防御化合物，我们在过表达 BoSULTR1 的植物中检测到胁迫耐受性;1/博舒尔特1;2.结果显示，BoSULTR1 的过表达;1/博舒尔特 1;拟南芥中的 2 显着增强了对 Helicoverpa armigera、细菌病原体丁香假单胞菌 pv 的抗性。 番茄 DC3000 和干旱胁迫。当 BoSULTR1;1/博舒尔特 1;2 在西兰花中过表达，表明 BoSULTR1 具有保守作用;1/博舒尔特 1;2 个在十字花科植物中。总之，BoSULTR1;1 和 BoSULTR1;2 个是增强芸苔科植物（如西兰花）硫吸收和提高抗逆性的有效候选基因。它们在农业生产中的潜在应用前景广阔。