Abiotic stresses, particularly drought and heat, pose significant threats to agricultural productivity and global food security. This study, therefore, investigated the responses of seven Prunus rootstocks (Garnem, GF 677, Marianna 2624, Myrobolan 29C, Rootpac 20, Rootpac 40, and Rootpac R) to drought, heat shock, and their combination, as well as their recovery potential. The present research encompassed a comprehensive analysis of some morphological, physiological, and biochemical parameters under controlled conditions. Our results revealed significant rootstock variations in stress tolerance and recovery potential. Rootpac 40 and Marianna 2624 consistently demonstrated superior tolerance across stress treatments, maintaining up to 30% higher relative shoot length and 25% higher stomatal conductance compared to other rootstocks under combined stress. Conversely, Rootpac 20 displayed heightened sensitivity, exhibiting up to 40% reduction in chlorophyll content and 50% increase in H2O2 levels under combined stress. GF 677 showed notable ABA accumulation, with a 6.9-fold increase under combined stress compared to control conditions. The combined drought and heat stress treatment induced significantly more severe impacts than individual stressors across all rootstocks. Proline accumulation increased by up to 9.3-fold under combined stress compared to 3–4 fold increases under individual stressors. Antioxidant enzyme activities were significantly upregulated under stress conditions, with Garnem, Rootpac 40, Rootpac R, and Marianna 2624 showing 3.6 to 5.1-fold increases under combined stress, depending on the specific enzyme. This comprehensive evaluation provided valuable insights into rootstock-specific stress response mechanisms and recovery potentials, offering a foundation for informed rootstock selection and breeding strategies aimed at enhancing stone fruit resilience under changing climatic conditions.

中文翻译：

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通过在干旱、热休克和综合胁迫条件下的综合评价来阐明李砧木的胁迫响应


非生物胁迫，尤其是干旱和高温，对农业生产力和全球粮食安全构成重大威胁。因此，本研究调查了 7 种李子砧木（Garnem、GF 677、Marianna 2624、Myrobolan 29C、Rootpac 20、Rootpac 40 和 Rootpac R）对干旱、热休克及其组合的反应，以及它们的恢复潜力。本研究包括在受控条件下对一些形态学、生理学和生化参数进行综合分析。我们的结果揭示了砧木在抗逆性和恢复潜力方面的显著差异。Rootpac 40 和 Marianna 2624 在胁迫处理中始终表现出卓越的耐受性，在联合胁迫下，与其他砧木相比，相对芽长高出 30%，气孔导度高出 25%。相反，Rootpac 20 表现出更高的灵敏度，在综合胁迫下，叶绿素含量降低高达 40%，H2O2 水平增加 50%。GF 677 显示出显着的 ABA 积累，与对照条件相比，在联合应激下增加了 6.9 倍。干旱和热应激联合处理对所有砧木的影响明显比单个应激源更严重。在联合压力下，脯氨酸积累增加了 9.3 倍，而在单个压力源下增加了 3-4 倍。抗氧化酶活性在胁迫条件下显著上调，Garnem、Rootpac 40、Rootpac R 和 Marianna 2624 在联合胁迫下增加 3.6 至 5.1 倍，具体取决于特定酶。 这项综合评估为砧木特异性胁迫反应机制和恢复潜力提供了宝贵的见解，为明智的砧木选择和育种策略提供了基础，旨在增强核果在变化气候条件下的恢复力。