Due to the lack of resistant germplasm, sclerotinia stem rot (SSR) caused by S. sclerotiorum (Sclerotinia sclerotiorum) has become the most destructive disease in rapeseed (Brassica napus L.) production. Nanobiotechnology has recently been considered as a sustainable approach for crop protection by reducing pesticide use. In this study, a novel blue-green fluorescent nanocomplex derived from licorice and wolfberry (LW-NCs) was synthesized and characterized, exhibiting stronger antioxidant activity compared with licorice or wolfberry extracts alone. LW-NCs promoted the growth of rapeseed seedlings without affecting photosynthesis efficiency. In vitro antifungal assays revealed that LW-NCs significantly inhibited the growth of S. sclerotiorum at a concentration of 200 ppm. Transcriptomic and metabolomic analyses demonstrated that foliar application of LW-NCs increased the JA content and the activities of SOD and CAT in rapeseed, ultimately enhancing the tolerance of rapeseed to S. sclerotiorum. In addition, toxicity experiments have demonstrated that LW-NCs possess excellent biocompatibility. These results indicated that the application of LW-NCs can not only improve the disease resistance of rapeseed, but also help reduce the harm of pesticides to human health, ensuring the safety of them for agricultural applications.

中文翻译：

---

甘草枸杞衍生的纳米材料通过激活 JA 介导的油菜籽免疫反应增强菌核茎腐病抗性


由于缺乏抗性种质，由菌核病 （Sclerotinia sclerotiorum） 引起的菌核病 （SSR） 已成为油菜籽 （Brassica napus L.） 生产中最具破坏性的病害。纳米生物技术最近被认为是一种通过减少杀虫剂使用来保护作物的可持续方法。在这项研究中，合成并表征了一种源自甘草和枸杞的新型蓝绿色荧光纳米复合物 （LW-NCs），与单独的甘草或枸杞提取物相比，表现出更强的抗氧化活性。LW-NCs促进了油菜幼苗的生长，而不影响光合作用效率。体外抗真菌试验显示，LW-NCs 在 200 ppm 浓度下显着抑制菌核链球菌的生长。转录组学和代谢组学分析表明，叶面喷施 LW-NCs 增加了油菜籽中的 JA 含量以及 SOD 和 CAT 活性，最终增强了油菜籽对菌核链球菌的耐受性。此外，毒性实验表明 LW-NCs 具有优异的生物相容性。这些结果表明，LW-NCs的应用不仅可以提高油菜籽的抗病性，还有助于减少农药对人体健康的危害，确保其农业应用的安全性。