Natural mucus-inspired hydrogels have recently garnered significant attention in the fields of drug delivery and tissue engineering. Herein, we successfully prepared mucin-based hydrogels with self-repairing capability and antioxidant activity by employing rational material design principles. By harnessing Bigineli multicomponent reaction and free radical polymerization, antioxidant dihydropyrimidin(thio)one (DHPM) and phenylboronic acid (PBA) groups were facilely integrated onto a zwitterionic copolymer (denoted as PMPD). Furthermore, leveraging the abundant glycans present on mucin allowed for dynamic cross-linking through phenylboronic ester bonds with PBA moieties of PMPD to fabricate PM hydrogels. The dynamic nature of phenylboronic ester endowed PM hydrogels with excellent properties such as self-healing, injectable, and self-adaptable. Moreover, owing to the presence of DHPM antioxidant groups along with intrinsic antioxidant activity of mucin, PM hydrogels showed exceptional efficacy in scavenging free radicals. Additionally, PM hydrogel, which was prepared using natural mucin biopolymer and PMPD copolymer mimicking cell membrane structure, showed favorable cytocompatibility. Therefore, our current study represents a novel design strategy for multifunctional mucus-inspired hydrogels that hold promising potential for biomedical applications.

中文翻译：

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自愈和抗氧化的粘液启发水凝胶


天然粘液启发的水凝胶最近在药物递送和组织工程领域引起了广泛关注。在此，我们采用合理的材料设计原理，成功制备了具有自我修复能力和抗氧化活性的粘蛋白基水凝胶。通过利用 Bigineli 多组分反应和自由基聚合，抗氧化剂二氢嘧啶（硫代）酮 （DHPM） 和苯硼酸 （PBA） 基团被轻松整合到两性离子共聚物（表示为 PMPD）上。此外，利用粘蛋白上存在的丰富聚糖，可以通过苯硼酯键与 PMPD 的 PBA 部分进行动态交联，以制备 PM 水凝胶。苯硼酸酯的动态特性赋予了 PM 水凝胶优异的性能，例如自修复、可注射和自适应性。此外，由于 DHPM 抗氧化基团的存在以及粘蛋白的内在抗氧化活性，PM 水凝胶在清除自由基方面表现出非凡的功效。此外，使用天然粘蛋白生物聚合物和模拟细胞膜结构的 PMPD 共聚物制备的 PM 水凝胶显示出良好的细胞相容性。因此，我们目前的研究代表了一种多功能粘液启发水凝胶的新型设计策略，具有广阔的生物医学应用潜力。