Cell-based delivery holds great promise for advancing cell therapy, but it often faces challenges such as low cell survival rates and immunological rejection during cell injection and circulation. In this study, we develop an alternative approach aimed at engineering cell membranes to produce encapsulated individual mammalian (EIM) cells. The encapsulation shell was formed by catalyzing the reaction of H2O2 with hyaluronic acid-dopamine (HA-DA) on the cell surface using horseradish peroxidase (HRP) enzymes. This protective shell not only protects live mammalian cells from physical stress but also from biological assaults. The individual cell encapsulation system enables the loading of cells with chemotherapy drugs, enhancing their targeting ability towards tumor sites and resulting in over 5.1-fold cell enrichment at metastatic tumors, thereby improving tumor-killing efficacy and reducing metastasis. Overall, the individual cell encapsulation system demonstrates potential for targeted drug delivery to the lungs in the treatment of lung cancer by reducing side effects and enhancing treatment outcomes.

中文翻译：

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将单个哺乳动物细胞封装为基于细胞的药物递送载体，用于治疗肺癌


基于细胞的递送为推进细胞疗法带来了巨大的前景，但它经常面临细胞存活率低和细胞注射和循环过程中的免疫排斥等挑战。在这项研究中，我们开发了一种替代方法，旨在设计细胞膜以产生封装的个体哺乳动物 （EIM） 细胞。通过使用辣根过氧化物酶 （HRP） 催化 H 2 O 2 与透明质酸-多巴胺 （HA-DA） 在细胞表面反应而形成包封壳。这种保护壳不仅可以保护活的哺乳动物细胞免受物理压力，还可以保护它们免受生物攻击。单个细胞包封系统能够用化疗药物加载细胞，增强它们对肿瘤部位的靶向能力，并在转移性肿瘤中使细胞富集超过 5.1 倍，从而提高肿瘤杀伤效果并减少转移。总体而言，单个细胞包封系统通过减少副作用和提高治疗结果，在肺癌治疗中显示出靶向药物递送到肺部的潜力。