Legumes are a predominant source of isoflavones, termed phytoestrogens, that mimic 17β-estradiol (E2). Phytoalexins are inducible isoflavones produced in plants subjected to environmental stressors (e.g., UV, heat, or fungi). This study investigated estrogenic activity of snow and green peas elicited with Aspergillus sojae. Elicited extracts increased estrogenic activity and proliferation of breast cancer cells (MCF-7 or T47D) in a dose-dependent manner but exhibited antiestrogenic activity when combined with synthetic E2. HPLC analysis of elicited pea extracts identified (+)-pisatin as the primary phytoalexin, which was produced significantly (p < 0.0001) more in snow pea compared to green pea. RNA sequencing results suggested potential functional effects on endothelial cells and tissue vascularization. Indeed, (+)-pisatin enhanced metrics of network assembly and maturation in a microphysiological model of bulk tissue vasculogenesis. Thus, context-dependent functional effects of (+)-pisatin and pharmacologically similar phytoestrogens on the entire tissue microenvironment should be considered in preclinical investigation as potential therapeutic agents.

中文翻译：

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在诱发的绿豌豆或荷兰豆 （pisum sativum） 中产生的 （+）-Pisatin 的环境依赖性雌激素作用


豆类是异黄酮的主要来源，称为植物雌激素，类似于 17β-雌二醇 （E2）。植物抗毒素是在受环境应激源（例如紫外线、热或真菌）的植物中产生的诱导型异黄酮。本研究调查了大豆曲霉引发的雪和青豌豆的雌激素活性。诱发的提取物以剂量依赖性方式增加乳腺癌细胞 （MCF-7 或 T47D） 的雌激素活性和增殖，但在与合成 E2 联合使用时表现出抗雌激素活性。对引出的豌豆提取物的 HPLC 分析确定 （+）-pisatin 是主要的植物抗毒素，与青豌豆相比，荷兰豆的产生量显着增加 （p < 0.0001）。RNA 测序结果表明对内皮细胞和组织血管形成的潜在功能影响。事实上，（+）-pisatin 增强了大组织血管发生的微生理模型中网络组装和成熟的指标。因此，在临床前研究中，应考虑 （+）-pisatin 和药理学上相似的植物雌激素对整个组织微环境的环境依赖性功能影响作为潜在的治疗剂。