This work introduces a fluorometric/electrical dual-biosensing logic system based on a DNA reaction network (DRN). This system was used to spatiotemporally modulate the kinetic behavior of DNA nanostructures. The system, acting as a programmable and modulative central controller introduced to implement, enabled the monitoring of the target gliotoxin. The DRN encompasses multiple pathways and provides a potential mechanistic way to develop dynamic networks that can evolve under directional controllable conditions. We demonstrated the implementation of a DRN to control the assembly and disassembly of a DNA conveyor belt. By exposing the responsive switches of the DNA conveyor belt, the DRN activates the operation of fluorescent DNA-driving axes based on the aggregation-induced emission effect, enabling signal generation and collection through continuous rolling on the surface of the DNA conveyor belt. The biosensor was employed to monitor gliotoxin, and under optimal conditions, dual-signal detection was achieved at 1.14 × 10^–7 and 2.45 × 10^–7 μg·mL^–1. The biosensor was integrated with a handheld electrochemical workstation, which enabled the successful monitoring of gliotoxin. This strategy enables self-tuning control and the multilayer hierarchical assembly of kinetic behaviors and is applicable to diverse fields such as biometric systems, medical diagnosis, and logic computing.

中文翻译：

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用于动态时空逻辑组装的 DNA 反应网络中央控制器及其在荧光/电生物传感合理设计中的应用


这项工作介绍了一种基于 DNA 反应网络 （DRN） 的荧光/电学双生物传感逻辑系统。该系统用于时空调节 DNA 纳米结构的动力学行为。该系统作为引入实施的可编程和调制中央控制器，能够监测目标胶质毒素。DRN 包含多种途径，并提供了一种潜在的机制方法来开发可以在定向可控条件下进化的动态网络。我们演示了 DRN 的实施来控制 DNA 传送带的组装和拆卸。通过暴露 DNA 传送带的响应开关，DRN 根据聚集诱导的发射效应激活荧光 DNA 驱动轴的操作，通过在 DNA 传送带表面连续滚动来产生和收集信号。采用生物传感器监测胶质毒素，在最佳条件下，在 1.14 × ^10-7 和 2.45 × ^10-7 μg^·mL-1 时实现了双信号检测。生物传感器与手持式电化学工作站集成，从而成功监测胶质毒素。该策略支持自调谐控制和动力学行为的多层分层组装，适用于生物识别系统、医学诊断和逻辑计算等不同领域。