Assisted reproductive technologies (ART) are pivotal for contemporary reproductive medicine and species conservation. However, the manual handling required in these processes introduces stress that can compromise oocyte and embryo quality. This study introduces OoTrap, a novel fluidic device designed to streamline ART workflows by facilitating the capture and maturation of oocytes in a compact unit. The device also reintroduces mechanical forces similar to those in the in vivo environment, which are often missing in conventional systems. OoTrap operates in both static and perfusion-based modes, offering flexibility and optimal conditions for oocyte maturation. Notably, OoTrap achieved higher in vitro maturation (IVM) rates under perfusion, produced oocytes with fewer chromosomal abnormalities, and maintained spindle morphology integrity. The incorporation of a heating system and a 3D-printed syringe pump enabled IVM outside the incubator, making OoTrap suitable for field applications. The results highlight the potential of OoTrap to enhance ART outcomes by reducing manual handling, providing a controlled microenvironment, and offering a practical solution for field-based ART applications.

中文翻译：

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OoTrap：使用可现场部署的流体装置增强卵母细胞收集和成熟。


辅助生殖技术 （ART） 对于当代生殖医学和物种保护至关重要。然而，这些过程中所需的手动操作会带来压力，从而影响卵母细胞和胚胎质量。本研究介绍了 OoTrap，这是一种新型流体装置，旨在通过促进卵母细胞在紧凑装置中的捕获和成熟来简化 ART 工作流程。该设备还重新引入了类似于体内环境中的机械力，这在传统系统中通常是缺失的。OoTrap 在静态和基于灌注的模式下运行，为卵母细胞成熟提供了灵活性和最佳条件。值得注意的是，OoTrap 在灌注下实现了更高的体外成熟 （IVM） 率，产生的卵母细胞染色体异常较少，并保持了纺锤体形态的完整性。加热系统和 3D 打印注射泵的结合使 IVM 能够在培养箱外进行，使 OoTrap 适用于现场应用。结果突出了 OoTrap 通过减少人工操作、提供受控微环境和为现场 ART 应用提供实用解决方案来增强 ART 结果的潜力。