The harnessing of microalgae for CO2 sequestration and biodiesel production has garnered significant interest due to its potential to mitigate greenhouse gas emissions and provide a renewable energy. The current research delving the dual applications of carbon sequestration efficiency and biodiesel synthesis competence of three microalgal strains of Chlorella vulgaris LCM605, Chlorococcum humicola LCM606, and Quadrigula closterioides LCM607 (hereafter Q. closterioides) isolated from coal mining site, Neyveli, India. Among the tested strains, the Q. closterioides exhibited high lipid content (37.65 % dwt) under CO2 supplementation and was selected in this study. CO2-tolerant microalgae were acclimated to high salinity concentration (100 mM) and enhanced lipid content of 50.94 (% dwt). The highest MUFA content (48.62–95.52 %) was observed in three strains, desirable for biodiesel production. Fuel quality was found to be complying with the limits of international biodiesel standards. To the best of our knowledge, this is the first report validating that coal mine microalgae Q. closterioides has the potential for sequestering CO2 and producing biodiesel under 15 % CO2. This research highlights the potential of Q. closterioides for CO2 sequestration and biodiesel production, emphasizing the importance of optimizing culture parameters for maximizing biomass and lipid yields. This study addresses the research gap in identifying microalgal species with dual functionality for CO₂ sequestration and biodiesel production, specifically targeting Q. closterioides from coal mine environment. By assessing its growth and lipid accumulation in this unique setting, the study explores its untapped potential for sustainable bioenergy applications.

中文翻译：

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释放煤矿微藻菌株的潜力：通过培养优化提高生物柴油产量和 CO₂ 封存


利用微藻进行 CO2 封存和生物柴油生产因其在减少温室气体排放和提供可再生能源方面的潜力而引起了人们的极大兴趣。目前的研究深入探讨了从印度 Neyveli 煤矿区分离的 Chlorella vulgaris LCM605、Chlorococcum humicola LCM606 和 Quadrigula closterioides LCM607 （以下简称 Q. closterioides） 三种微藻菌株的碳封存效率和生物柴油合成能力的双重应用。在供试菌株中，梭形栎在 CO2 补充下表现出高脂质含量 （37.65 % dwt），并在本研究中被选中。耐 CO2 的微藻适应了高盐度浓度 （100 mM） 和高达 50.94 （% dwt） 的脂质含量。在三种菌株中观察到最高的 MUFA 含量 （48.62–95.52%），是生物柴油生产的理想选择。发现燃料质量符合国际生物柴油标准的限制。据我们所知，这是第一份验证煤矿微藻 Q. closterioides 具有封存 CO2 和生产 15% CO2 以下生物柴油的潜力的报告本研究强调了 Q. closterioides 在 CO2 封存和生物柴油生产方面的潜力，强调了优化培养参数以最大限度地提高生物量和脂质产量的重要性。本研究解决了在鉴定具有 CO₂ 封存和生物柴油生产双重功能的微藻物种方面的研究空白，特别是针对煤矿环境中的 Q. closterioides。通过评估其在这种独特环境中的生长和脂质积累，该研究探索了其在可持续生物能源应用方面尚未开发的潜力。