Background The declaration of mpox as a Public Health Emergency of International Concern highlights the need for interventions to interrupt virus transmission, including transmission via fabrics. Current World Health Organization guidance on clothes washing is based on a general consensus of virus inactivation; however, there is uncertainty about the efficacy of laundry detergents and disinfectants or the reduction of risk achieved by washing clothes for monkeypox virus (MPXV) specifically. Methods This study investigates the efficacy of manual washing for inactivating MPXV from clothes. Using a simulated washing method, we evaluated the efficacy of commonly-used laundry products and high temperature water for inactivating MPXV on fabrics. Cotton and polyester fabrics were inoculated with MPXV for 1 min, placed in a microcentrifuge tube containing water or water with test product for 20 minutes, with agitation every 5 min to simulate manual washing. Results Sodium hypochlorite, liquid sanitizer, and two powdered laundry detergents dissolved in room temperature water, as well as 70-degree water alone, completely inactivated MPXV (>3 log10reduction or >99.9% inactivation) on both cotton and polyester fabrics. Conclusion Given the expected concentrations of MPXV on fabrics, the low transfer rate of viruses from porous surfaces to skin, the effective inactivation of laundry processes, and the expected doses required for infection, we expect the risk of transmission after laundering contaminated fabrics to be low. This study provides evidence to support WHO guidance for MPXV inactivation, reducing the viral load on fabrics to prevent the spread of mpox in both healthcare and household settings.

中文翻译：

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洗衣方法从织物中消除猴痘病毒 （MPXV） 的功效


背景 宣布 mpox 为国际关注的突发公共卫生事件强调了采取干预措施以阻断病毒传播的必要性，包括通过织物传播。目前世界卫生组织关于衣物洗涤的指导方针基于病毒灭活的普遍共识;然而，洗衣粉和消毒剂的功效或专门针对猴痘病毒 （MPXV） 洗涤衣物所实现的风险存在不确定性。方法 本研究调查了手动洗涤对灭活衣物中 MPXV 的疗效。使用模拟洗涤方法，我们评估了常用洗衣产品和高温水灭活织物上 MPXV 的功效。将棉和涤纶织物接种 MPXV 1 分钟，置于盛有水或水的微量离心管中与供试品共 20 分钟，每 5 分钟搅拌一次，模拟手动洗涤。结果 次氯酸钠、液体消毒剂和两种洗衣粉溶解在室温水中，以及单独的 70 度水中，棉和涤纶织物上的 MPXV 完全灭活（>3 log10reduction 或 >99.9% 灭活）。结论鉴于织物上 MPXV 的预期浓度、病毒从多孔表面到皮肤的低传播率、洗衣过程的有效灭活以及感染所需的预期剂量，我们预计洗涤受污染织物后的传播风险很低。这项研究提供了证据来支持 WHO 关于 MPXV 灭活的指南，减少织物上的病毒载量，以防止 mpox 在医疗保健和家庭环境中传播。