Conducting polymer hydrogels (CPHs) are ideal for biomolecule detection due to their porous structure allowing rapid diffusion of gases and liquids, along with their inherent conductivity, flexibility, and biocompatibility. However, their limited catalytic efficiency can be improved by incorporating noble metal nanoparticles (NMNPs), which enhance electron transfer, structural stability, and catalytic activity, making them more effective for applications in bioelectronics, sensors and related applications. Herein, we report the two terminal device with active channel formed by the incorporation of platinum nanoparticles into polyaniline-polyvinyl alcohol (Pt-NPs@PANI/PVA) hydrogel for glucose biomolecule detection. The structural and morphological characterization of Pt-NPs@PANI/PVA hydrogel confirmed the presence of Pt-NPs within the PANI-PVA hydrogel matrix. The sensor exhibited an enhanced electrical conductivity while showing the reduced hydrophilicity in presence of Pt-NPs. Though the sensor demonstrated the lesser liquid absorption compared to PANI/PVA hydrogel but conducted a significant current in DI water media. Furthermore, it also displayed the electrical responsiveness in tear fluid. The sensor response was measured for glucose biomolecule after functionalizing with glucose oxidase, (GOx) and preconditioning with DI water media. The sensor sensitivity to glucose was 7.364 μA/dec within the linear range 1 μM–30 mM with the detection limit 0.44 μM. The sensor exhibited good reproducibility highlighting its potential for development of wearable sensors in various health care monitoring, aiming to provide a low cost, user friendly solution for glucose biomarker detection with promising applications in non-invasive health monitoring.

中文翻译：

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铂包埋导电聚苯胺/聚乙烯醇水凝胶，用于增强葡萄糖生物分子检测


导电聚合物水凝胶 （CPH） 是生物分子检测的理想选择，因为它们的多孔结构允许气体和液体快速扩散，以及其固有的导电性、柔韧性和生物相容性。然而，它们有限的催化效率可以通过掺入贵金属纳米颗粒 （NMNP） 来提高，从而增强电子传递、结构稳定性和催化活性，使其在生物电子学、传感器和相关应用中的应用更加有效。在此，我们报道了通过将铂纳米颗粒掺入聚苯胺-聚乙烯醇 （Pt-NPs@PANI/PVA） 水凝胶中用于葡萄糖生物分子检测而形成的具有活性通道的双末端装置。Pt-NPs@PANI/PVA 水凝胶的结构和形态学表征证实了 PANI-PVA 水凝胶基质中存在 Pt-NPs。该传感器表现出增强的导电性，同时在存在 Pt-NP 的情况下显示出降低的亲水性。尽管与 PANI/PVA 水凝胶相比，传感器的液体吸收较少，但在去离子水介质中传导了很大的电流。此外，它还显示了泪液中的电反应性。用葡萄糖氧化酶 （GOx） 功能化并用去离子水培养基预处理后，测量传感器对葡萄糖生物分子的响应。在 1 μM–30 mM 的线性范围内，传感器对葡萄糖的灵敏度为 7.364 μA/dec，检测限为 0.44 μM。该传感器表现出良好的重现性，突出了其在各种医疗保健监测中开发可穿戴传感器的潜力，旨在为葡萄糖生物标志物检测提供低成本、用户友好的解决方案，在无创健康监测中具有广阔的应用前景。