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Root exudates regulate soil antibiotic resistance genes via rhizosphere microbes under long-term fertilization
Environment International ( IF 10.3 ) Pub Date : 2024-12-11 , DOI: 10.1016/j.envint.2024.109180 Xiujing Guan, Yuhui Li, Yanying Yang, Zihua Liu, Rongguang Shi, Yan Xu
Environment International ( IF 10.3 ) Pub Date : 2024-12-11 , DOI: 10.1016/j.envint.2024.109180 Xiujing Guan, Yuhui Li, Yanying Yang, Zihua Liu, Rongguang Shi, Yan Xu
Organic fertilizer application promotes the prevalence of antibiotic resistance genes (ARGs), yet the factors driving temporal differences in ARG abundance under long-term organic fertilizer application remain unclear. This study investigated the temporal dynamics of ARG diversity and abundance in both bulk and rhizosphere soils over 17 years (2003–2019), and explored microbial evolution strategies, ARG hosts succession and the influence of root exudates on ARGs regulation. The results showed that the ARGs abundance in rhizosphere soil was lower than that in bulk soil under long-term fertilization, and ARGs abundance exhibited a decrease and then remained stable in rhizosphere soil over time. There was a strong association between host bacteria and dominant ARGs (p < 0.05). Structural equations demonstrated that bacterial community had a most pronounced influence on ARGs (p < 0.05), and metabolites exhibited an important mediation effect on bacterial community (p < 0.05), thereby impacting ARGs. The metabolome analysis evidenced that significant correlations were found between defensive root exudates and most ARGs abundance (p < 0.05), like, luteolin-7-glucoside was negatively correlated with tetA(58). These findings provide deeper insights into the dynamics of soil ARGs under long-term fertilization, and identify critical factors that influence ARGs colonization in soils, providing support for controlling the spread of ARGs in agriculture soils.
中文翻译:
根系分泌物在长期施肥下通过根际微生物调控土壤抗生素抗性基因
有机肥施用促进了抗生素抗性基因 (ARGs) 的流行,但在长期施用有机肥下导致 ARG 丰度时间差异的因素仍不清楚。本研究调查了 17 年 (2003-2019) 块状和根际土壤中 ARG 多样性和丰度的时间动态,并探讨了微生物进化策略、ARG 宿主演替和根系分泌物对 ARGs 调节的影响。结果表明:长期施肥后,根际土壤中ARGs丰度低于块状土壤,且根际土壤中ARGs丰度随时间推移呈下降趋势后保持稳定。宿主细菌与显性 ARGs 之间存在很强的关联 (p < 0.05)。结构方程表明,细菌群落对 ARGs 的影响最显着 (p < 0.05),代谢物对细菌群落表现出重要的介导作用 (p < 0.05),从而影响 ARGs。代谢组分析证明,防御性根分泌物与大多数 ARGs 丰度之间存在显着相关性 (p < 0.05),例如,木犀草素-7-葡萄糖苷与 tetA 呈负相关 (58)。这些发现为长期施肥下土壤 ARGs 的动态提供了更深入的见解,并确定了影响 ARGs 在土壤中定植的关键因素,为控制 ARGs 在农业土壤中的传播提供了支持。
更新日期:2024-12-11
中文翻译:
根系分泌物在长期施肥下通过根际微生物调控土壤抗生素抗性基因
有机肥施用促进了抗生素抗性基因 (ARGs) 的流行,但在长期施用有机肥下导致 ARG 丰度时间差异的因素仍不清楚。本研究调查了 17 年 (2003-2019) 块状和根际土壤中 ARG 多样性和丰度的时间动态,并探讨了微生物进化策略、ARG 宿主演替和根系分泌物对 ARGs 调节的影响。结果表明:长期施肥后,根际土壤中ARGs丰度低于块状土壤,且根际土壤中ARGs丰度随时间推移呈下降趋势后保持稳定。宿主细菌与显性 ARGs 之间存在很强的关联 (p < 0.05)。结构方程表明,细菌群落对 ARGs 的影响最显着 (p < 0.05),代谢物对细菌群落表现出重要的介导作用 (p < 0.05),从而影响 ARGs。代谢组分析证明,防御性根分泌物与大多数 ARGs 丰度之间存在显着相关性 (p < 0.05),例如,木犀草素-7-葡萄糖苷与 tetA 呈负相关 (58)。这些发现为长期施肥下土壤 ARGs 的动态提供了更深入的见解,并确定了影响 ARGs 在土壤中定植的关键因素,为控制 ARGs 在农业土壤中的传播提供了支持。