Cell membrane proteins are important components used to study cellular life processes and for clinical diagnostics. Currently, the signal of membrane protein imaging is limited to the pivot of the target, significantly limiting signal amplification efficiency and output, unlike the efficiency observed in solution systems. Inspired by the widespread recruitment phenomenon in natural life activities, here, we propose a molecular dynamic recruitment technique for the amplified imaging of cell membrane proteins. It overcomes the limitations of imaging based on the target protein as a pivot point, allowing the signal at the pivot point to diffuse across the entire cell membrane, significantly enhancing signal amplification capability. To achieve this goal, firstly, two types of DNA are labeled respectively to the cell membrane and membrane proteins. Then, the strand displacement of the effector DNA triggers molecular dynamic recruitment based on an entropy-driven circuit, recruiting a large number of probes from the solution to the cell membrane, thus realizing the amplified imaging of membrane proteins. In summary, we have constructed a novel platform for amplified imaging of membrane protein, providing a new perspective for the application of cell diagnostics.

中文翻译：

---

受自然启发的分子动态募集，用于细胞膜蛋白的扩增成像


细胞膜蛋白是用于研究细胞生命过程和临床诊断的重要成分。目前，膜蛋白成像的信号仅限于靶标的枢轴，与在溶液系统中观察到的效率不同，这严重限制了信号放大效率和输出。受自然生命活动中广泛募集现象的启发，我们在这里提出了一种分子动力学募集技术，用于细胞膜蛋白的放大成像。它克服了以靶蛋白为枢轴点成像的局限性，允许枢轴点的信号扩散到整个细胞膜上，从而显着增强信号放大能力。为了实现这一目标，首先，将两种类型的 DNA 分别标记到细胞膜和膜蛋白上。然后，效应 DNA 的链位移触发基于熵驱动回路的分子动态募集，从溶液中募集大量探针到细胞膜，从而实现膜蛋白的扩增成像。综上所述，我们构建了一种新的膜蛋白扩增成像平台，为细胞诊断的应用提供了新的视角。