The skin wound is susceptible to bacterial invasion, which hinders the healing of the wound, especially when infected with multi-drug resistant strains. This demands novel bioactive materials to combat bacterial infections. In this study, gallium oxide nanoparticles (Ga₂O₃ NPs) were successfully synthesized through high-temperature thermal decomposition, exhibiting excellent biocompatibility and photocatalytic antimicrobial activity. The Ga₂O₃ NPs were crosslinked into chitosan hydrogel to create a light-responsive multilayered 3D porous hydrogel (Ga₂O₃ NPs hydrogel) for use in photocatalytic antimicrobial therapy (PCAT). The prepared Ga₂O₃ NPs hydrogel exhibits broad-spectrum photocatalytic activity and remarkable antibacterial efficacy against E. coli and S. aureus. It effectively eradicates biofilms, promotes reactive oxygen species production, disrupts bacterial cell membranes, and induces nucleic acid leakage, ultimately resulting in bacterial death. Additionally, it exhibits excellent biosafety. Both in vitro pigskin and in vivo mouse wound infection models have confirmed the remarkable efficacy of Ga₂O₃ NPs hydrogel in PCAT. Notably, Ga₂O₃ NPs hydrogel created a moist environment for the wound in an MDR S. aureus-infected mouse wound model, demonstrating significant potential to facilitate wound healing and minimize scar formation. This study introduces a novel hydrogel dressing without antibiotic components for resistant bacterial-infected wounds.

中文翻译：

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一种用于光催化抗菌治疗的光响应多层 3D 多孔 Ga2O3 水凝胶，可促进 MDR 金黄色葡萄球菌感染伤口的愈合


皮肤伤口易受细菌侵袭，阻碍伤口愈合，尤其是感染多重耐药菌株时。这需要新型生物活性材料来对抗细菌感染。在本研究中，通过高温热分解成功合成了氧化镓纳米颗粒 （Ga₂O₃ NPs），表现出优异的生物相容性和光催化抗菌活性。将 Ga₂O₃ NPs 交联到壳聚糖水凝胶中，形成光响应多层 3D 多孔水凝胶 （Ga₂O₃ NPs 水凝胶），用于光催化抗菌治疗 （PCAT）。制备的 Ga₂O₃ NPs 水凝胶表现出广谱光催化活性，对大肠杆菌和金黄色葡萄球菌具有显著的抗菌效果。它有效根除生物膜，促进活性氧的产生，破坏细菌细胞膜，并诱导核酸泄漏，最终导致细菌死亡。此外，它还表现出优异的生物安全性。体外猪皮和体内小鼠伤口感染模型都证实了 Ga₂O₃ NPs 水凝胶在 PCAT 中的显着疗效。值得注意的是，在 MDR 金黄色葡萄球菌感染的小鼠伤口模型中，Ga₂O₃ NPs 水凝胶为伤口创造了潮湿的环境，显示出促进伤口愈合和减少疤痕形成的巨大潜力。本研究介绍了一种不含抗生素成分的新型水凝胶敷料，用于治疗耐药细菌感染的伤口。