Cell transfer printing plays an essential role in biomedical research and clinical diagnostics. Traditional bioadhesion-based methods often necessitate complex surface modifications and offer limited control over the quantity of transferred cells. There is a critical need for a modification-free, non-labeling, and high-throughput cell transfer printing technique. In this study, an adhesion-free cellular transfer printing method based on vibration-induced microstreaming is introduced. By adjusting the volume of the microcavity, the number of cells transferred per microtiter well can be realized to the level of a single cell. Additionally, it allows for precise control of large-scale cellular spatial distribution, leading to the formation of biomimetic patterns. Moreover, the demonstrated biocompatibility and high throughput of this cell transfer printing method highlight its potential utility. The correspondence of the transferred cell amount to the vibration and frequencies allows the system to exhibit excellent tunability of the transferred cell amount and pattern. This bioadhesion-free cell transfer printing method holds promise for advancing cell manipulation in biomedical research and analysis.

中文翻译：

---

推进细胞转移打印：通过振动微流实现无生物粘附沉积。


细胞转移打印在生物医学研究和临床诊断中起着至关重要的作用。传统的基于生物粘附的方法通常需要复杂的表面修饰，并且对转移细胞的数量控制有限。迫切需要一种无需修饰、无标记和高通量细胞转移打印技术。在本研究中，介绍了一种基于振动诱导微流的无粘附细胞转移打印方法。通过调整微腔的体积，每个微量滴定孔转移的细胞数量可以实现到单个细胞的水平。此外，它允许精确控制大规模细胞空间分布，从而形成仿生模式。此外，这种细胞转移打印方法的生物相容性和高通量突出了其潜在用途。转移的电池量与振动和频率的对应关系使系统能够表现出转移电池量和模式的出色可调性。这种无生物粘附的细胞转移打印方法有望在生物医学研究和分析中推进细胞操作。