As the field of neural organoids and assembloids rapidly expands, there is an emergent need for guidance and advice on designing, conducting and reporting experiments to increase the reproducibility and utility of these models. Here, our consortium– representing specialized laboratories from around the world– presents a framework for the experimental process that ranges from ensuring the quality and integrity of human pluripotent stem cells to characterizing and manipulating neural cells in vitro, and from transplantation techniques to considerations for modeling human development, evolution, and disease. As with all scientific endeavors, we advocate for rigorous experimental designs tailored to explicit scientific questions, and transparent methodologies and data sharing, to provide useful knowledge for both current research practices and for developing regulatory standards.

随着神经类器官和组装体领域的迅速扩展，迫切需要有关设计、实施和报告实验的指导和建议，以提高这些模型的可重复性和实用性。在这里，我们的联盟（代表来自世界各地的专业实验室）提出了一个实验过程框架，范围从确保人类多能干细胞的质量和完整性到在体外表征和操作神经细胞，从移植技术到模拟人类发育、进化和疾病的考虑因素。与所有科学努力一样，我们倡导针对明确的科学问题量身定制的严格实验设计，以及透明的方法和数据共享，为当前的研究实践和制定监管标准提供有用的知识。