Insecticide resistance is a pressing issue in urban pest management. The annual bluegrass weevil (ABW), Listronotus maculicollis Kirby, is a highly destructive turfgrass pest in northeastern North America. The overuse of pyrethroids has led to resistance in some ABW populations in the northeastern United States. Understanding ABW resistance mechanisms is pivotal for sustaining turf quality and minimizing environmental impact. This study investigated the potential for enzymatic detoxification, a common resistance mechanism in insects, to be the mechanism behind ABW tolerance to pyrethroids. Different ABW populations’ resistance levels to bifenthrin were determined through dose–response Petri dish assays. Cytochrome P450 activity and protein concentrations were measured following bifenthrin exposure. Results indicated golf course‐specific effects on enzyme and protein levels but found no direct correlation between these levels and bifenthrin dosage. P450 activity varied among different golf course populations, showing no dependence on bifenthrin dosage. These findings question prior studies suggesting P450 involvement in bifenthrin detoxification in populations of all resistance levels. This study establishes a foundation for comprehending ABW resistance mechanisms and devising effective management strategies. However, further research is needed to investigate additional detoxification enzymes and other resistance mechanisms.

中文翻译：

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对拟除虫菊酯耐药 Listronotus maculicollis Kirby 解毒酶的评估提示其他机制


杀虫剂耐药性是城市害虫管理中的一个紧迫问题。一年生蓝草象鼻虫 （ABW） Listronotus maculicollis Kirby 是北美东北部的一种极具破坏性的草坪草害虫。拟除虫菊酯的过度使用导致美国东北部一些 ABW 种群产生耐药性。了解 ABW 抗性机制对于维持草坪质量和最大限度地减少环境影响至关重要。本研究调查了酶解毒（昆虫中常见的耐药机制）成为 ABW 对拟除虫菊酯耐受性机制的潜力。通过剂量反应培养皿测定确定不同 ABW 群体对联苯菊酯的耐药水平。暴露后测量细胞色素 P450 活性和蛋白质浓度 联苯菊酯结果表明高尔夫球场特异性对酶和蛋白质水平的影响，但发现这些水平与联苯菊酯剂量之间没有直接相关性。P450 活性因高尔夫球场的不同人群而异，对联苯菊酯剂量没有依赖性。这些发现质疑了先前的研究表明 P450 参与所有抵抗水平人群的联苯菊酯解毒。本研究为理解 ABW 耐药机制和制定有效的管理策略奠定了基础。然而，需要进一步的研究来研究其他解毒酶和其他耐药机制。