BACKGROUNDGray mold in tomato caused by Botrytis cinerea is a destructive disease, which can be treated using biocontrol agents. Pretreatment of biocontrol strains with oxidative‐stress‐ameliorating compounds can enhance their tolerance to oxidative microenvironment in infected fruit wounds. In this study, we aimed to determine the effect of caffeic acid (CA) on the biocontrol efficacy of Pantoea jilinensis D25 in gray mold in cherry tomato.RESULTSD25 strain pretreated with CA exhibited enhanced oxidative stress response in cherry tomato wounds. CA‐treated D25 cells recovered from cherry tomato wounds had lower ROS level and cellular oxidative damage than D25 cells not treated with CA. The antioxidation‐related genes (CAT and GPX) were upregulated and colonization ability of D25 strain was improved in cherry tomato wounds after CA application. Furthermore, the biocontrol effects of CA‐treated D25 strain were improved. CA pretreatment to D25 strain could significantly improve the expression of defense‐related genes (SlMYC‐2, SlLoxD, SlEIN2, and SlEIN3) and activity of defense‐related enzymes in cherry tomato wounds.CONCLUSIONApplication of CA to D25 strain could improve its ability to combat antioxidant stress and biocontrol efficacy against postharvest tomato gray mold. © 2024 Society of Chemical Industry.

中文翻译：

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咖啡酸提高 Pantoea jilinensis D25 对番茄灰霉病的生防效果


背景灰霉菌引起的番茄中的霉菌是一种破坏性疾病，可以使用生物防治剂进行治疗。用氧化应激改善化合物预处理生防菌株可以提高它们在受感染的水果伤口中对氧化微环境的耐受性。在本研究中，我们旨在确定咖啡酸 （CA） 对樱桃番茄灰霉病 D25 生防效果的影响。RESULTSD25 用 CA 预处理的菌株在樱桃番茄伤口中表现出增强的氧化应激反应。与未用 CA 处理的 D25 细胞相比，从樱桃番茄伤口中恢复的 CA 处理的 D25 细胞具有更低的 ROS 水平和细胞氧化损伤。CA 施用后，抗氧化相关基因 （CAT 和 GPX） 上调，D25 菌株在樱桃番茄伤口中的定植能力提高。此外，CA 处理的 D25 菌株的生防效果得到改善。对 D25 菌株进行 CA 预处理可显著提高樱桃番茄伤口防御相关基因 （SlMYC-2 、 SlLoxD 、 SlEIN2 和 SlEIN3） 的表达和防御相关酶的活性。结论施用 CA 到 D25 菌株可以提高其对抗抗氧化应激的能力和对采后番茄灰霉病的生防效果。© 2024 化工学会.