Yeast strains have been widely recognized as useful cell factories for biomanufacturing. To improve production efficiency, their biosynthetic pathways and regulatory strategies have been continuously optimized. However, commercial production using yeasts is still limited by low product yield and high production cost. Accumulating evidences have demonstrated the importance of metabolite transport processes in addressing these challenges. Engineering yeast membrane transporters for transporting precursors, substrates, intermediates, products and toxic inhibitors has been successful. In addition, membrane properties are also important for metabolite production. Here we propose membrane transport engineering (MTE) to integrate manipulation of both membrane transporters and membrane properties. We emphasize that systematic optimization of both transporters and membrane lipid bilayers benefits production efficiency. We also envision the potential of artificial intelligence and automation process in MTE for economic and sustainable bioproduction using yeast cell factories.

中文翻译：

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用于高效酵母生物制造的膜转运工程


酵母菌株已被广泛认为是生物制造的有用细胞工厂。为了提高生产效率，他们的生物合成途径和调节策略不断得到优化。然而，使用酵母的商业生产仍然受到产品产量低和生产成本高的限制。越来越多的证据表明，代谢物转运过程在应对这些挑战方面的重要性。用于运输前体、底物、中间体、产物和毒性抑制剂的工程酵母膜转运蛋白已经成功。此外，膜特性对于代谢物的产生也很重要。在这里，我们提出了膜转运工程 （MTE） 来整合膜转运蛋白和膜特性的操作。我们强调转运蛋白和膜脂质双层的系统优化有利于生产效率。我们还设想了人工智能和自动化过程在 MTE 中的潜力，以使用酵母细胞工厂进行经济和可持续的生物生产。