Polyhydroxyalkanoate (PHA) is considered a sustainable alternative to traditional petroleum-based plastics due to its biodegradability and biocompatibility. In this study, Acidovorax diaphorobacter ZCH-15, an efficient PHA-producing strain, was isolated from activated sludge. Using food waste-derived orange peel as a substrate, the strain initially achieved a PHA concentration of 0.39 g/L. Under optimal fermentation conditions (30℃, pH 8, 2 % inoculum concentration, and 30 g/L carbon source), the PHA concentration increased by 138 % to reach a maximum of 0.93 g/L. Proton nuclear magnetic resonance spectroscopy and gas chromatography analyses identified the PHA composition as poly(3-hydroxybutyrate-co-3-hydroxyvalerate), which exhibited high crystallinity and structural stability. Metabolomic analysis indicated that the tricarboxylic acid cycle and pentose phosphate pathway were involved in producing succinyl-CoA, a precursor required for PHA synthesis. This study demonstrates the potential for cost-effective industrial PHA production while enabling the high-value utilization of food waste.

中文翻译：

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使用桔皮从新分离的 Acidovorax diaphorobacter ZCH-15 分离细菌中生产聚羟基脂肪酸酯及其潜在的代谢机制


聚羟基脂肪酸酯 （PHA） 因其生物降解性和生物相容性而被认为是传统石油基塑料的可持续替代品。在本研究中，从活性污泥中分离出一种高效的 PHA 产生菌株 Acidovorax diaphorobacter ZCH-15。使用食物垃圾衍生的橙皮作为基质，该菌株最初达到 0.39 g/L 的 PHA 浓度。在最佳发酵条件 （30°C、pH 8、接种浓度 2 % 和碳源 30 g/L） 下，PHA 浓度增加了 138 %，最高达到 0.93 g/L。质子核磁共振波谱和气相色谱分析确定 PHA 组成为聚（3-羟基丁酸酯-co-3-羟基戊酸酯），表现出高结晶度和结构稳定性。代谢组学分析表明，三羧酸循环和磷酸戊糖途径参与产生琥珀酰辅酶 A，琥珀酰辅酶 A 是 PHA 合成所需的前体。这项研究展示了具有成本效益的工业 PHA 生产的潜力，同时实现了食物垃圾的高价值利用。