Light-harvesting chlorophyll a/b-binding (Lhcb) proteins play key roles in light energy capture and transfer, leaf development, plant growth, and various stress responses. However, their role in Wucai (Brassica campestris L.) has not yet been studied. In this study, 27 Lhcb genes were identified in Brassica rapa. Chromosomal location analysis revealed that these 27 genes are distributed on nine chromosomes. Subcellular localization analysis indicated that many BrLhcb proteins were present in the chloroplast. The number of exons and introns in BrLhcb family genes was in the range of 1–7 and 0–6, respectively. Cis-acting element analysis of the BrLhcb promoters demonstrated that light-responsive elements were the most common along with elements related to stress and stress-related hormonal signals. In addition, an evolutionary analysis indicated a closer relationship between Lhcb genes of B. rapa and A. thaliana compared with the other three Brassica species. Through transcriptome data analysis and quantitative reverse transcription polymerase chain reaction (RT-qPCR), we identified a crucial gene, BcLhcb2.1, which is localized in chloroplasts and is highly expressed in leaves. Overexpression of BcLhcb2.1 enhanced cold, salt, and drought tolerance in Arabidopsis. This study provides a theoretical basis for exploring the functions of the BrLhcb gene family and for identifying and screening stress-resistance genes.

中文翻译：

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BcLhcb2.1 是一种来自 Wucai 的光捕获叶绿素 a/b 结合蛋白，在响应非生物胁迫中起正调节作用


光捕获叶绿素 a/b 结合 （Lhcb） 蛋白在光能捕获和转移、叶片发育、植物生长和各种胁迫反应中起关键作用。然而，它们在五彩 （Brassica campestris L.） 中的作用尚未得到研究。在这项研究中，在甘蓝中鉴定出 27 个 Lhcb 基因。染色体位置分析显示，这 27 个基因分布在 9 条染色体上。亚细胞定位分析表明，许多 BrLhcb 蛋白存在于叶绿体中。BrLhcb 家族基因中的外显子和内含子数量分别在 1-7 和 0-6 的范围内。BrLhcb 启动子的顺式作用元件分析表明，光响应元件与压力和压力相关的激素信号相关元件是最常见的。此外，进化分析表明，与其他三种芸苔属植物相比，B. rapa 和 A. thaliana 的 Lhcb 基因之间的关系更密切。通过转录组数据分析和定量逆转录聚合酶链反应 （RT-qPCR），我们鉴定了一个关键基因 BcLhcb2.1，该基因位于叶绿体中，在叶片中高表达。过表达 BcLhcb2.1 增强了拟南芥的耐寒、耐盐和耐旱性。本研究为探索 BrLhcb 基因家族的功能以及鉴定和筛选抗逆基因提供了理论依据。