The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and N,N-methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic groups. The material's hydrophilicity was further enhanced by a ring-opening reaction with cysteine (the product was denoted as Cys-PVEM). Finally, titanium sulfate was combined with Cys-PVME to form titanium-rich polymers (Cys-PVME-Ti⁴⁺) for the enrichment of phosphopeptides and glycopeptides. Cys-PVME-Ti⁴⁺ has a good sensitivity (0.02 fmol) and selectivity (1 : 1000) with a loading capacity of 62 mg g⁻¹, recyclability (9 cycles), and a good recovery rate (101.6 ± 0.60%) for phosphopeptides, and good sensitivity (0.01 fmol μL⁻¹), selectivity (1 : 2000), a loading capacity of 62.5 mg g⁻¹, recyclability (9 cycles), and a good recovery rate (98.7 ± 1.2%) for glycopeptides. In addition, after enrichment with this material, 27 phosphopeptides with 14 phosphoproteins and 223 glycopeptides associated with 88 glycoproteins were captured from the serum of colorectal cancer patients, while 27 phosphopeptides associated with 14 phosphoproteins and 210 glycopeptides associated with 111 glycoproteins were also captured from the serum of a normal control. Gene ontology (GO) analysis revealed that complement activation, extracellular region, extracellular space, blood coagulation, the IgG immunoglobulin complex, and heparin binding were different between normal control and colorectal cancer, implying that related pathways are likely involved in colorectal cancer pathogenesis.

中文翻译：

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制备富含亲水基团的钛官能化聚合物材料，用于血清中的磷酸化蛋白质组和糖化蛋白质组分析


蛋白质磷酸化和糖基化分析对于研究疾病发展至关重要。在这项工作中，1,2-环氧-5-己烯和 N，N-亚甲基双丙烯酰胺与磷酸乙烯基聚合，生成包含多种亲水基团的聚合物（表示为 PVME）。通过与半胱氨酸的开环反应（该产物表示为 Cys-PVEM）进一步增强了该材料的亲水性。最后，硫酸钛与 Cys-PVME 结合形成富钛聚合物 （Cys-PVME-Ti⁴⁺），用于富集磷酸肽和糖肽。Cys-PVME-Ti⁴⁺ 具有良好的灵敏度 （0.02 fmol） 和选择性 （1 ： 1000），载量为 62 mg ^g-1，可回收性（9 个循环）和良好的磷酸肽回收率 （101.6 ± 0.60%），以及良好的灵敏度 （0.01 fmol ^μL-1）、选择性 （1 ： 2000），载样量为 62.5 mg ^g-1、可回收性（9 次循环）和糖肽的良好回收率（98.7 ± 1.2%）。此外，用这种材料富集后，从结直肠癌患者的血清中捕获了 27 个磷酸肽和 223 个与 88 个糖蛋白相关的糖肽，同时从正常对照的血清中捕获了 27 个与 14 个磷酸蛋白相关的磷酸肽和 210 个与 111 个糖蛋白相关的糖肽。 基因本体论 （GO） 分析显示，正常对照癌和结直肠癌的补体激活、细胞外区域、细胞外间隙、血液凝固、IgG 免疫球蛋白复合物和肝素结合不同，表明相关通路可能参与结直肠癌发病机制。