BACKGROUNDHolometabolan pupal‐specifier broad‐complex (BR‐C) and adult specifier ecdysone‐induced protein 93F (E93) are essential for metamorphosis; however, their interaction and effects on programmed cell death and cell differentiation during pupation remain unclear.RESULTSHere, multiple single‐guide RNA (sgRNA)‐mediated mosaic knockout of BR‐C induced a deformed larva/pupa intermediate phenotype in Spodoptera frugiperda. Quantitative real‐time polymerase chain reaction (qPCR) analysis showed that the adult specifier E93 was prematurely expressed in the BR‐C mutants during the penultimate and last instar larval stages. Additionally, histological observation and TUNEL assay showed that apoptosis in the fat body and midgut was activated in the larval tissues; astonishingly, the adult midgut appeared in the pupae of BR‐C mutants.CONCLUSIONOverall, the results demonstrated that the premature expression of E93 induced by lack of BR‐C triggers adult differentiation during the larval stages, which revealed the inhibitory effect of BR‐C on E93 during metamorphosis in S. frugiperda. © 2024 Society of Chemical Industry.

中文翻译：

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敲除 BR-C 诱导 E93 的过早表达，从而触发幼虫形态下的成体分化


背景 Holometabolan 蛹特异性宽复合物 （BR-C） 和成体特异性蜕皮激素诱导的蛋白 93F （E93） 对至关重要;然而，它们在化蛹过程中对程序性细胞死亡和细胞分化的相互作用和影响仍不清楚。结果多个单向导 RNA （sgRNA） 介导的 BR-C 马赛克敲除诱导了草地贪夜蛾中变形的幼虫/蛹中间表型。定量实时聚合酶链反应 （qPCR） 分析表明，成虫特异性 E93 在倒数第二龄和最后一龄幼虫阶段在 BR-C 突变体中过早表达。此外，组织学观察和 TUNEL 测定显示，幼虫组织中脂肪体和中肠的细胞凋亡被激活;令人惊讶的是，成年中肠出现在 BR-C 突变体的蛹中。结论总体而言，缺乏 BR-C 诱导的 E93 过早表达触发了幼虫期的成体分化，这揭示了 BR-C 对草地贪夜蛾过程中 E93 的抑制作用。© 2024 化工学会.