BACKGROUNDDiaphorina citri Kuwayama is the main vector of the Huanglongbing (HLB) pathogen Candidatus Liberibacter asiaticus (CLas). Therefore, reducing CLas acquisition by D. citri is crucial for controlling the spread of HLB.RESULTSThis study aimed to investigate the effect of ZnO quantum dots (ZnO QDs) on CLas acquisition by D. citri. The results showed that ZnO QDs were absorbed by citrus plants and D. citri, inducing reactive oxygen species generation in the intestine and causing intestinal cell apoptosis. The uptake rate of CLas was reduced to 75% and 22.3% after treatment with 2 and 5 mg ∙ mL−1 ZnO QDs, respectively, compared with 100% in the control group. Transcription analysis showed significant changes in the expression of D. citri genes related to apoptosis, oxidative stress, and DNA replication and repair.CONCLUSIONThe results suggested that ZnO QDs could efficiently reduce CLas acquisition by D. citri, providing a safe and reliable tool for HLB prevention and control. © 2024 Society of Chemical Industry.

中文翻译：

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ZnO 量子点减少 Diaphorina citri 对黄龙病原菌的获取


背景Diaphorina citri Kuwayama 是黄龙病 （HLB） 病原体 Candidatus Liberibacter asiaticus （CLas） 的主要载体。因此，减少柠檬果树对 CLas 的获取对于控制 HLB 的传播至关重要。结果本研究旨在探讨 ZnO 量子点 （ZnO QDs） 对柑橘 D. 获得 CLas 的影响。结果表明，ZnO QDs 被柑橘类植物和柑橘 D. 吸收，诱导肠道活性氧生成并引起肠道细胞凋亡。与对照组的 100% 相比，用 2 mg 和 5 mg ∙ mL-1 ZnO QD 处理后，CLas 的摄取率分别降低到 75% 和 22.3%。转录分析显示，与细胞凋亡、氧化应激以及 DNA 复制和修复相关的 D. citri 基因表达发生显著变化。结论 ZnO QDs 可有效减少柠檬果树对 CLas 的获取，为 HLB 防控提供了安全可靠的工具。© 2024 化工学会.