Phenolic compounds are typical organic pollutants which cause severe human health problems due to their teratogenesis, carcinogenesis, neurotoxicity, immunotoxicity and endocrine disruption. Natural laccase is a multicopper oxidase existing in bacteria, plants, and insects, which can accelerate the transformation of phenolic compounds to their less hazardous oxidized products under mild conditions without harmful byproducts. Despite eco-environmentally friendly property of laccase, it still faces constraints of widespread application attribute to its high cost, complex preparation, and vulnerability. Therefore, exploring laccase mimics with high catalytic activity attracts a lot of attention and endeavors. In this research, copper-based nanozymes were prepared with coordination of copper ions and imidazole for mimicking the active sites of natural laccase via solvothermal method. The obtained Cu-based (Cu-Im) nanozymes exhibited multiple redox valence states of Cu and laccase-mimicking coordination structures, which endow Cu-Im with high laccase-like activity. During the process of catalytic oxidation reactions, singlet oxygen and superoxide anions generated from oxygen. Encouraged by the catalytic property, Cu-Im was utilized in degradation and detection of phenolic and catecholamine compounds. The catalytic degradation of compounds by Cu-Im showed good conversion and substrate versatility, which can be used as a kind of potential materials for phenolic pollutant degradation and remediation. Simultaneously, colorimetric sensors of phenols and catecholamines based on Cu-Im in solution system and POCT pad platform were constructed which indicated wide linear range and low limit of detection for both detection strategies. The Cu-Im-based sensor was a promising method for sensitive, fast, convenient, and qualitative-quantitative colorimetric analysis of phenols and catecholamines. The outcomes of this research elucidate Cu-Im is a satisfactory substitute for natural laccase, which will have broad application prospects in laccase-related fields, such as environmental recovery, pollution monitoring, and diagnosis of neurological diseases etc.

中文翻译：

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活性位点启发多铜漆酶样纳米酶，用于检测酚类和儿茶酚胺化合物


酚类化合物是典型的有机污染物，由于其致畸、致癌作用、神经毒性、免疫毒性和内分泌干扰，会导致严重的人类健康问题。天然漆酶是一种存在于细菌、植物和昆虫中的多铜氧化酶，可以在温和的条件下加速酚类化合物转化为危害较小的氧化产物，而不会产生有害的副产物。尽管漆酶具有生态环保特性，但由于其成本高、制备复杂和脆弱性，它仍然面临广泛应用的限制。因此，探索具有高催化活性的漆酶模拟物吸引了大量的关注和努力。在这项研究中，用铜离子和咪唑的配位制备了铜基纳米酶，用于通过溶剂热法模拟天然漆酶的活性位点。获得的 Cu 基 （Cu-Im） 纳米酶表现出 Cu 和漆酶模拟配位结构的多个氧化还原价态，赋予 Cu-Im 高漆酶样活性。在催化氧化反应过程中，由氧生成单线态氧和超氧阴离子。在催化特性的鼓励下，Cu-Im 被用于酚类和儿茶酚胺化合物的降解和检测。Cu-Im催化降解化合物表现出良好的转化率和底物通用性，可作为一种潜在的酚类污染物降解和修复材料。同时，构建了基于 Cu-Im 的溶液系统和 POCT 垫平台的酚类和儿茶酚胺比色传感器，表明两种检测策略的线性范围宽，检测限低。 基于 Cu-Im 的传感器是一种很有前途的方法，可用于对酚类和儿茶酚胺进行灵敏、快速、方便和定性定量比色分析。本研究结果阐明 Cu-Im 是天然漆酶的令人满意的替代品，在漆酶相关领域具有广阔的应用前景，如环境恢复、污染监测和神经系统疾病诊断等。