Microwell technology is crucial in biological applications due to its ability to handle small sample sizes and perform numerous assays efficiently. This study aimed to develop a novel technique for microwell fabrication using pressure-assisted steam technology, offering lower cost, simplicity, and high reproducibility. Mechanical properties of microwell surfaces were successfully controlled and characterized, making them suitable for DNA capture. The application of gold coating generated an electric field within designed microwells, facilitating stable DNA detection. These microwells exhibited effective DNA sensing capabilities, validated using fluorescently stained lambda DNA at various concentrations (86, 8.6, and 0.86 ng μL-1). In particular, the 2.8 mm microwell showed a greater change in fluorescence intensity depending on DNA concentration than other microwells. At a concentration of 0.86 ng μL-1, to assess producibility using relative standard deviation (RSD) values as a DNA sensor, they were measured as 5.29, 2.76, and 1.85% for 1, 1.7, and 2.8 mm microwells, respectively. These results indicated that our proposed microwell exhibited efficient performance and good reproducibility. We believe that the developed method could be potentially used for high-throughput analysis as a biosensor for DNA applications.

中文翻译：

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受控 Au 包被的 PDMS 微孔阵列，用于表面增强的 DNA 生物芯片。


Microwell 技术在生物应用中至关重要，因为它能够处理小样品量并高效执行多种分析。本研究旨在开发一种使用压力辅助蒸汽技术制造微孔的新技术，具有成本低、简单性和高重现性的特点。成功控制和表征了微孔表面的机械特性，使其适合于 DNA 捕获。金涂层的应用在设计的微孔内产生电场，有助于稳定的 DNA 检测。这些微孔表现出有效的 DNA 传感能力，使用不同浓度（86、8.6 和 0.86 ng μL-1）的荧光染色 lambda DNA 进行验证。特别是，与其他微孔相比，2.8 mm 微孔显示出更大的荧光强度变化，具体取决于 DNA 浓度。在 0.86 ng μL-1 浓度下，使用相对标准偏差 （RSD） 值作为 DNA 传感器评估生产性，1 mm、1.7 mm 和 2.8 mm 微孔的检测结果分别为 5.29%、2.76% 和 1.85%。这些结果表明，我们提出的微井表现出高效的性能和良好的可重复性。我们相信，开发的方法有可能用于高通量分析，作为 DNA 应用的生物传感器。