Piezo ion channels play a role in bladder sensation, but the sensory afferent subtypes that utilise Piezo channels have not been fully explored. We made single-unit extracellular recordings from mucosal-projecting bladder afferents in guinea pigs with protamine/zymosan-induced cystitis. The Piezo1 agonist, Yoda1, significantly potentiated mechanosensitivity, while its antagonist, Dooku1, abolished this potentiation. The P2 purinoceptor antagonist, PPADS abolished α,β-methylene ATP-induced excitation of mucosal afferents without affecting their mechanical activation or potentiation of mechanosensitivity by Yoda1. The findings suggest Piezo1, but not ATP, is required for mechanotransduction in bladder mucosal afferents in cystitis.

中文翻译：

---

Piezo1 而不是 ATP 是膀胱炎中膀胱粘膜传入神经机械转导所必需的


压电离子通道在膀胱感觉中发挥作用，但利用压电通道的感觉传入亚型尚未得到充分探索。我们从患有鱼精蛋白/酵母聚糖诱导的膀胱炎的豚鼠的粘膜突出膀胱传入神经中进行了单单位细胞外记录。Piezo1 激动剂 Yoda1 显着增强了机械敏感性，而其拮抗剂 Dooku1 消除了这种增强。P2 嘌呤受体拮抗剂 PPADS 消除了 α，β-亚甲基 ATP 诱导的粘膜传入神经兴奋，而不影响 Yoda1 对其机械激活或机械敏感性的增强。研究结果表明，在膀胱炎中，膀胱粘膜传入神经的机械转导需要 Piezo1，而不是 ATP。